Chemical And Activity Study Of Hericium Erinaceur Oligosaccharide

Hericium erimaceus,classified into Basidioycotina,belongs to Holobasidiomycetydae in Hymenomyates.It is not only a kind of domestic fungus,but also can be used in pharmaceutics.Basic research and clinical application proves its reliable pharmaceutical action,however,concerning about its oligosaccharide contents,there is few reports about this aspect.The author of this article mainly carried out detailed research in this field.The ethanol precipitation extracted by watering and precipitating the Hericium contained 61.70%of sachride the dialysis of 3500 MWt membrane, Hep could be separated into two parts:Hep-1 and Hep-2,which contained 76.3%and 23.7%of sachride,respectively.Hep-1 was further analyzed to contain Hep-1-1 and Hep-1-2 by the DEAE-Sephadex A-50 column chromatography.However,the sachride in the former part occupied 84.87%. The molecular weight of Hep-1-1 distributed between 197 and 1800.After the removal of the floating protein by Sevag method,it was demonstrated,by its physical and chemical characteristics that proteins of Hep-1-1 bind to the carbohydrates.In the pharmaco-experiments,Hep-1-1 can cure the chronic atrophic gastritis to some extent,which proved the medical value of its Oligosaccharide.After the column chromatography of absorbite powder,Hep-1-1 were separated into five parts:Tp-1,Tp-2,Tp-3,Tp-4,Tp-5.Sachride occupied 88.15%of Hep-1-1-3.Through the MWt analysis,the molecular weight distributed between 123-1750.Tp-3 was formed the mixture of pentosaccharide,hexasaccharide,and heptasaccharide revealed by MS test.After the repeated absorbite column chromatography,Tp-3 were further separated into another four parts:Hep-1-1-3-1,Tp-3-2,Tp-3-3 and Tp-3-4. The third part contained 94.31%Oligosaccharide.Following acetylation,GC analysis confirmed Tp-3-2 was consisted of Glu. Revealed by MS analysis,Tp-3-2 was composed by the mixture of pentosaccharide,hexasaccharide,heptasaccharide,octasaccharide and nonasaccharide.In GC-MS analysis,partially methylated Tp-3-2 was demonstrated its neutral glucosidic bond:1→4 glucosidic bond with branches at the third and sixth locus.