Expression And Relationship Between DNA TopoⅡ And CD44v6 In Gastric Carcinoma

Chemotherapy and surgical operation have been two main means and obtained obvious effect in gastric carcinoma treatments.The clinical curative effect of gastric carcinoma is not satisfactory because of chemotherapeutic agents resistance and invasion of tumor cells.Multidrug resistance(MDR)is one of the main reasons which causes chemotherapy failure,DNA topoisomeraseⅡ(TopoⅡ)is the target point for some cytotoxic drugs,and is also the important target enzyme of MDR,which mediate the MDR of many chemotherapeutics.CD44 is a kind of transmembrane glycoprotein existing in cell surface.CD44v6 is a splicing variant of CD44 mediating lymphocyte's homing, participating in the adhesion action among cells,which play a important role in tumor transference and invasion.Demonstration about the relationship between DNA TopoⅡand MDR could be seen in many articles,but we have not seen few reports about relationship in MDR,metastasis and invasion for gastric carcinoma.The aim of tiffs study is to examine the expression of DNA TopoⅡand CD44V6 and their relationship in gastric carcinoma.Objective:To examine the expression and significance of DNA TopoⅡand CD44v6 and their relationship with invasion and metastasis for gastric carcinoma.Methods:Immunohistochemistry SP method was used to determine the expression of DNA TopoⅡand CD44v6 in carcinoma mucosa and normal mucosa(4cm away from carcinoma edge)in 86 tumor samples from the patients with gastric carcinoma.Date was analyzed by chi-squie test.Results:The positive expression rates of DNA TopoⅡand CD44v6 in gastric carcinoma were 40.69%and 50%,respectively.The higher expression ofDNA TopoⅡwas significantly associated with lymph nodes metastasis and differentiation degree(P＜0.05), and had no correlation to infiltration depth(P＞0.05).The higher expression of CD44v6 was significantly associated with lymph nodes metastasis and infiltration depth(P＜0.05), andhad no correlation to differentiation degree(P＞0.05).The positive expression rates of CD44v6 in DNA TopoⅡpositive expression group were remarkably higher than that in TopoⅡnegative expression group.Conclusion:Both DNA TopoⅡand CD44v6 play important role in gastric carcinoma development and progression.DNA TopoⅡexpression may be a useful predictor of MDR, and is relevant to differentiation degree and metastasis in gastric carcinoma.The expression of CD44v6 may be a useful marker for predicting the metastasis potency.MDR has significantly correlation to infiltration depth in gastric carcinoma.Detecting expression of DNA TopoⅡand CD44v6 might reflect the clinical biology behavior and guide the chemotherapy and surgery for gastric carcinoma.Sensitive chemotherapy medicine may inhibit or reverse the metastasis potency for gastric carcinoma.