Correlation Between The Expression Of RhoA/Rho Kinase System In Uterine Smooth Muscle During Pregnant And The Onset Of Labor

Objective:To investigate the role of RhoA/Rho kinase system in the onset of labor.Methods:20 cases in labor(L group).20cases not in labor(NL group) but in term pregnancy,and 20 cases of non-pregnant(NP group)women undergoing hysterectomy due to cervical intraepithelial neoplasia were recruited into this clinical study.Real-time quantitative polymerase chain reaction and Western Blotting methods used to investigate the expression of RhoA and ROCKI mRNA and the corresponding protein.Results:1)The expression of RhoAmRNA in L group[(3.51±0.56)×10^-4]was significantly higher than that in NL group[(2.81±0.52)×10^-4],P＜0.05,which was significantly higher than that in NP group[(2.11±0.54)×10^-4],P＜0.05.2)The expression of ROCKI mRNA in L group was[(4.07±0.66)×10^-3]was significantly higher than that in NL group[(2.71±0.31)×10^-3],P＜0.05,which was significantly higher than that in NP[(2.01±0.23)×10^-3],P＜0.05.3)The expression of RhoA protein in the L group,NL group,NP group were(0.72±0.13),(0.64±0.17),(0.58±0.14).There was not significantly difference in among different groups,P＞0.05.4)The expression of ROCKI protein in L group (0.56±0.14),was significantly higher than that in NL group(0.42±0.11), P＜0.01,which was significantly higher than that in NP group(0.29± 0.08),P＜0.01.Conclusion:The expression and activity of RhoA/Rho kinase in the uterine smooth muscle may play an important role in the onset of labor.