| Objective:To study the effect of hydrogen sulfide(H2S)on the size of single colonic smooth muscle cell in vitro and to investigate whether the effect of H2S on colonic smooth muscle cell size is mediated by ATP-sensitive potassium channel(KATP channel).So that to know how H2S works on gastrointestinal motility and what is its molecule mechanism.Methods:.The single colonic smooth cells suspension of Wister rats were prepared. Sodium hydrosulfide(NaSH)(H2S donor),both NaSH and Glibenclamide(Gli) (KATPchannel inhibitor),and Pinacidil(Pin)(KATPchannel opener)were added in cells suspension respectively.Cells were observed under the inverted phase contrast microscope and the sizes were measured with an image analysis system to evaluate the effects of drugs.Results:1.In NaSH group,the cells were longer at 60 seconds than the cells at 30 or 120 seconds(P＜0.05)and they were also longer than the control group(P＜0.05), with a relaxation value of 20.48%.So,an action time of 60 seconds was employed in the later experiments.2.The cells added by NaSH at 1×10-5,1×10-4and 1×10-3mol/L were longer than the control group(P＜0.05).The cells treated with NaSH at 1×10-3and 1×10-4mol/L were longer than those treated with NaSH at 1×10-5mol/L (P＜0.05).No statistical significance was found in the length of cells between the group treated with NaSH at 1×10-4mol/L and the group treated with NaSH at 1×10-3mol/L(P＞0.05).3.The cells treated with NaSH + 1×10-6mol/L Gli,NaSH + 1×10-5mol/L Gli, NaSH + 1×10-4mol/L Gli,NaSH + 1×10-3mol/L Gli were all shorter than the cells treated with NaSH +PBS and longer than the cells treated with PBS +PBS(the control group)(P＜0.05).Negative correlation was found between the Gli concentration and the cell length.As the Gli concentration was increased from 1×10-6to 1×10-4mol/L,the average cell length were becoming shorter (P＜0.05).There was no statistical significance in the cell length between the cells treated with NaSH + 1×10-4mol/L Gli and those treated with NaSH+ 1×10-3mol/L Gli(P＞0.05).4.The cells were longer in Pin group(1×10-6,1×10-5and 1×10-4mol/L respectively)than the control group(P＜0.05).The cell length increased as the concentration of Pin increased.Conclusion:1.Cells could be relaxed by NaSH.NaSH with an action time of 60 seconds had the largest relaxation effect on the colonic smooth cells.In addition,a dose-dependent relaxation effect could be founed.In a certain dosage range,the relaxation effect enhanced as the concentration of NaSH increased.2.The relaxation effect of cells induced by NaSH could be attenuated by Gli and a dose-dependent inhibition effect could be found.But the relaxation effect of NaSH could not be blocked by Gli completely.3.Pin had the same effect on cells relaxation as NaSH.4.H2S might produce the relaxation effect on colonic smooth muscle cells of wister rats,which mightbe partly due to the activation of KATPchannel.It could be inferred that H2S was probably involved in the regulation of the gastrointestinal motility as a gaseous signal molecule. |
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