| Objective: Placental growth factor ( PlGF ) is a member of the vascular endothelial growth factor (VEGF)family of growth factors. PlGF stimulates vascular smooth muscle cell growth, recruits macrophages into atherosclerotic lesions, up-regulates production of tumor necrosis factor and monocyte chemotactic protein -1 by macrophages, stimulates the inflammtory reaction. It plays a fundamental role for unstable inflammation plaque of atherosclerosis.Simultaneously,CRP also plays a fundamental role for unstable inflammation plaque of atherosclerosis as a inflammation factor. Thus, furture research of PlGF and Hs-CRP will elevate the recogniaion of early and advanced atherosclerotic lesions. Furthermore, we will investigate the role of PlGF on the development of acute myocardial infarction (AMI).Methods: 60 patients with acute myocardial infarction (AMI), who came from the inpatients of 2th hospital affiliated with the HEBEI Medical University ( 33 males and 27 females ), with average age of (56.4±8.0) years. The patients with AMI were divided into two groups : the thrombolysis group ( 29 males and 6 females ) with average age of (52.8±7.2) years and the non-thrombolysis group ( 18 males and 7 females ) with average age of (51.8±9.1) years. 30 healthy staffs were recruited as normal control group. All subjects not only had excluded body mass index ( BMI )≥25kg/m2 and <18.5kg/m2, but also had no other diseases such as Cerebrovascular disease, Main Artery disease, Liver and Kidney disease, Metabolic disease ( Diabetes, Hyperthyreosis ). The age, sex , BMI showed no difference among three groups. Taking ulnar venous blood 5ml on an empty stomach, infusing anticoagulation tube, using centrifugal machine, 3000 r /min, centrifuging 10min, the plasma in Eppendorf was preserved in -80℃refrigerator. Serum placental growth factor (PlGF) were detected by enzyme-linked immuneosorbent assay (ELISA), while the levels of Hs-CRP were detected by immunity transmission turbidity. Cardiac ultrasonography were detected on 14 days of onset, the relationship between adiponectin and the markers of Serum placental growth factor, Hs-CRP was analyzed.Results: The level of Serum placental growth factor at 12hours and the 3days and 7 days were respectively (17.47±4.85) pg/mL, (20.47±5.42) pg/mL, (9.30±2.89) pg/mL, significantly raised than control group (7.56±1.37) pg/mL (P<0.01) except the 7 days group, the level of Serum Hs-CRP at the same time were respectively (33.57±32.18) mg/L, (41.76±10.19) mg/L, (28.03±8.34)mg/L, significantly raised than control group (1.5±2.0) mg/L(P<0.05). The level of placental growth factor was positively correlated with that of CK-MB (r=0.131;P>0.05) and Hs-CRP was positively correlated with that of CK-MB (r=0.364;P<0.01), while placental growth factor was positively with Hs-CRP ( r=0.014;P>0.05). The level of placental growth factor was negatively correlated with left ventricular ejection (LVEF) (r=-0.654;P<0.01), and positive correlation with left ventricular end-diastolic volume(LVEDV) (r= 0.845; P<0.01).Conclusion: 1 The level of serum PlGF and Hs-CRP at 12hours and the 3days and 7 days were significantly raised than control group,and the 3 days were the highest,suggested that PlGF and Hs-CRP contribute to the pathophysiological process of aute myocardial infarction; 2 The level of serum PlGF and Hs-CRP of the thrombolysis group were significantly raised than that of the nonthrombolysis group,suggested that the level of serum PlGF and Hs-CRP were affected by reperfusion ; 3 The level of serum PlGF was positively correlated with that of CK-MB ,and the level of Hs-CRP was positively correlated with that of CK-MB while PlGF was positively with Hs-CRP , suggested that PlGF related to myocardial injury area; 4 The level of PlGF was negatively correlated with LVEF, and positive correlation with LVEDV,suggested that PlGF may influence left ventricular remodeling.
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