Preparation Of Clobetasol Propionate Liposome And Its Pharmacodynamics Study On Guinea Pig Eczema Model

OBJECTIVE:To prepare clobetasol propionate liposome and to investigate its stability and to study its promoting effects on percutaneous permeation in vitro diffusion experiment and pharmacodynamics on Guinea Pig Eczema Model so as to provide a theoretical basis for clinical application.METHODS.1 clobetasol propionate liposome was prepared with revolution thin film dispersion,The indices such as the content of the principal agent,grain size,encapsulation efficiency and its stability were investigated.2 Permeability of CP liposome through full-thickness guinea pig abdorminal skin in vitro were performed,with CP cream as control,to investigate the enhancement of skin permeation of CP liposome.3 The guinea pig model of chronic eczema was established.The pharmacodynamics of CP liposome and cream on Guinea Pig Eczema Model were studied by evaluating cellular infiltrates in dermis.RESULTS:0.01%(W/V)CP liposome was prepared successfully.The content of the principal agent,grain size and encapsulation efficiency of liposome were(100.84±2.0)%,(140.5nm%56.8nm)nm, (90.1±2.3)%,respectively.No obvious changes were found in all the indices in the stability of liposome under three different storage condition. In vitro diffusion experiment,deposition amount in dermis of CP through geniue pig abdorminal skin was markedly higher in CP liposome group than that in commercial CP cream group(2.65±0.23ug/g vs 0.42±0.15ug/g,P＜0.05).Cellular infiltrates in dermis of CP liposome group and commercial CP cream group were 12.72±2.61/HPF and 22.94±2.33/HPF,espectively with significant difference(P＜0.05).CONCLUSION:The preparation was proved to be of high encapsulation efficiency,good stability.The permeability through skin is better with CP liposome than that with commercial CP cream in vitro diffusion experiments.3.Upon pharmacodynamical results,CP liposome has better curative effect than CP cream.