Study Of ERK₂ By Methods Of Immunohistochemistry And In Situ Hybridization In Lesions Of Psoriasis

Psoriasis is a kind of chronic, inflammatory , hyperplastic skin disease. Proliferation of keratinocyte (KC),infiltration of inflammatory cell and angiopoiesis are the three most important elements of its histological character. It affects patients' physical and mental health at a high incidence, for a long course and with frequent recurrence. The pathogenesis of psoriasis is still unknown. Previous researches indicated that the signal transduction pathway of the mitogen-activated protein kinase(MAPK) plays a critical role in cell growth,development,differentiation ,proliferation,apoptosis and the regulation of functions between cells. Whether its function is normal or not,Which may be related with proliferation of psoriatic epidermal KC. Researches indicated that ERK's activate is related with cells' proliferation ,and ERK₂ is more active than ERK₁ in the process of cell growth and differentiation .ERK₂ protein levels were assessed by immunohistochemical methods and its mRNA levels were detected by means of in situ hybridization in lesions of psoriasis. The aim of this study is to detect their correlation,research the relationship between ERK₂ and proliferation of psoriatic KC.Results:l.the result of immunohistochemical methods indicated that the expression of ERK₂ in psoriasis vulgaris was significantly higher than that of normal skins;2. the result of means of in situ hybridization indicated that the expression of ERK₂ in psoriasis vulgaris was significantly higher than that of normal skins;3.statistics indicated two parts had significant differences.Conclusion:ERK₂ was over expressed in psoriasis vulgaris, which hinted that ERK₂ plays an important role in the development of psoriasis and takes part in the proliferation of psoriatic epidermal KC.