Studies On Chemical Constituents And Fingerprint Spectrum For Active Fraction Of Chloranthus Japonicus Sieb.

Chloranthus japonicus Sieb is a kind of perennial herbaceous plant, which belongs to the family of Chloranthaceae.Chloranthus japonicus Sied has been widely used to treat cold rheum,injuries from falls,fracture, rheumatism ache,pulmonary tuberculosis,neurasthenic,impaludism,etc.in the civilian society of China.It mainly grows in shady places,including hillside,valley,tussock,etc.Chloranthus japonicus Sieb has comprehensively distributed in many places in China,including Heilongjiang,Jilin,Hebei, Shanxi,Shandong,Shanxi,Gansu,etc.Chloranthus japonicus Sieb is very rich in the plant resources in China.However,for the long time,this plant has Not been exploited and utilized availably by people.For better illumination medical substance basis on treating cancer,we have studies on chemical constituents of 50%ethaNol elution section of macroporous absorption resin for Chloranthus japonicus Sieb by silica gel, ODS,Sephadex LH-20 column chromatography and HPLC preparation chromatography.As a result,chemical structures of 6 compounds were fully determined by 1D and 2D NMR such as ¹H-NMR,¹³C-NMR,DEPT, ¹H-¹HCOSY,HSQC and HMBC,etc,combined with ESI-MS and HR-ESI-MS, UV,IR and chemical methods.The compounds are one lignans (cleomiscosin C-4-O-β-glucopyraNoside),two sesquiterpences(pisumio No side and 1α-hydroxyeudesman-4(15),7(11)-dien-8α-12-olide-l-O- glucopyra Noside),two saponin compounds(CaulosideD,LenticinD)and one coumarins (kimmin-7-O-glucopyra Noside).Proper method for the determination of HPLC fingerprint spectrum of Chloranthus japonicus Sieb was established and up to the technical demands for TCM fingerprint spectrums.HPLC fingerprint spectrums of different places and sources of Chloranthus japonicus Sieb were determined and 20 common fingerprint peaks were asertained.Similarities of the fingerprint spectrums were compared and showed that their compenents were similar.This dissertation elucidated the anti-tumor material basis of Chloranthus japonicus Sieb by chemical and Fingerprint Spectrums research. It will provide important scientific foundation for further research and the development of new drugs and promote the exploitation of this abundant natural medicinal resource.