| Objective:Chronic Pancreatitis(CP)is a kind of commonly seen digestive disease,which typical pathological representation is fabrosis of pancreatic texture.So anti-fibrous of pancreatic texture is the key point to CP. This experiment proves that the therapy of "invigorating spleen and promoting blood by regulating qi" is very important in the anti-fibrous of pancreatic texture treatment process by observing the contents of MDA and the expression of TNF-αand PDGF-B.Method:The research based on basic theory of TCM,set up the treatment principle of "invigorating spleen and promoting blood by regulating qi",and constituted "YI TAI Compounded Medication",then take experiments; Our research injected L-Arg into abdomen of the rats to make spleen deficiency rat model,it was a new model which combine diseases and syndromes;Utilizing the techniques,such as immuno-histochemistry,optical microscope and Elisa,the paper investigated the effect of the regulating factors of texture TNF-αand PDGF-B's expression and MDA's contents of serum in Chronic Pancreatitis rats;Meanwhile,observes the intervening effect of "YI TAI Compounded Medication".This paper studied the anti-fibrous mechanism from tissue form and molecular level.Result:1.The treatment principle of "invigorating spleen and promoting blood by regulating qi" could recovers most of the pancreas acinar,reduces fiber constituents in pancreas lobular,accelerate absorption of inflammatory cells,increase body's dissolved ability;2.The treatment principle of "invigorating spleen and promoting blood by regulating qi" could restrains the expression of TNF-αand PDGF-B,decreases the contents of MDA,so as to reduces the deposition of ECM and reverses fibrosis.Conclusion:The treatment principle of "invigorating spleen and promoting blood by regulating qi" is an effective method of preventing and treating CP,and YI TAI Compounded Medication has a higher clinical value by reducing the contents of MDA and restraining the expression of TNF-αand PDGF-B. |
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