To Observe The Affection Of Wugongbaiduyin Decoction Towards The Mitosis Of Vaginal Epithelial Cells Of Mouse In The Estrogen Period

Objective To observe the affection of WUGONGBAIDUYIN decoction towards the mitosis of vaginal epithelial cells of mouse in the estrogen period.Methods 50 KM mouse,female,with intraperitoneal injection of Diethylstilbestrol for3 days,are induced in estrogen period.They are divided into 5 groups randomly. Group 1 is treated with WGBDY decoction( 8.78g/kgd), Group 2 is treated with WGBDY decoction(17.5 5 g/kg·d) ,Group 3 is treated with WGBDY decoction ( 35.10g/kg·d) ,Group4 is treated with suspension of FUFANGQINGDAI capsule, Group 5 is treated with NS.All the medicine are given by gavage,at 8Am,for 7 days.On the 8^th day,at 10 Am, all the 5 groups are treated with Colchicine by intraperitoneal injection 15mg/kg, to make the mitosis stagnated in the Medium-term for count.Kill the mouse,cut the vaginal organizations,and make the pathological section.Observe the cells in mitosis among the basal cells with optical microscope and count.Results In group 5,there are more cells in mitosis. Nucleus increase and deep.In other groups,the amount of cells in mitosis decrease.When group 1,2,3,4 are compared with group 5, P<0.01.When group 3 is compared with group 4, P<0.05.Conclusion WGBDY decoction inhibit the mitosis of vaginal epithelial cells of mouse.