Study On Chitosan Nanoparticles As The Carrier For Cytokine Sustained-Release Preparation

Chitosan come from the shell of shrimp and crab. In our country, the resources of chitosan is abundant, the yield is high, and the price is low. It is a kind of alkalescence amylase that existed in the nature exclusively, no poisonous and biodegradable. In the last few years chitosan conduct and actions as the new medicine complements is in the ascendant. Sustaine-release preparations is a new product with many advantage. It includes accurate curative effect, badly respond to reduce, patients compliance. Sustained–release preparations used chitosan is not only function of sustained -release markednessly, but also can't accumulate in the body.Chitosan-BSA is prepared by ioncrosslinking in this paper. The preparation method is mild without organic solvent, so it is suitable for keeping the integrity and biological stability of the albumen and polypeptide. Nanoparticles from 50nm to 683nm are prepared in this paper by modifying the parameter. It determined loading efficiency of chitosan-BSA sustained-release nanoparticles is 58.45% by UV. The content of CS is 0.3%, the content of TPP is 0.15%, Chitosan:BSA =2:1,pH is 5.1-6.0 and time of stirring is 30 min. It shows that the diameter is uniform and the dispersion is good by SEM. The in vitro release test showed that the effects of the drug loading, chitosan content, TPPcontent and freeze drying on the release rate were much.Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine which exhibits activity in the hematopoietic and immune systems. GM-CSF was originally cloned based on its ability to stimulate the proliferation and differentiation of hematopoetic precursors of the granulocyte monocyte lineage. The serum elimination half-life of human GM-CSF in humans is approximately one hour, and therefore daily injections are required to achieve therapeutic effects, To induce the frequency of GM-CSF administration and improve the therapeutic efficency, Chitosan-GM-CSF sustained-release nanoparticles was prepared. It shows the diameter of GM-CSF is less than 200nm by TEM. It determined drug loading of chitosan-GM-CSF sustained-release nanoparticle is 155.01ug/100mg by SPF. The in vitro release test showed that GM-CSF-CS-NPS released fast in 1 hour and became slower after 7 hour.