Expression Of Soluble Endoglin And Other Angiogenic Factors In Peripheral Blood Of Preeclampsia

OBJECTIVETo investigate the diagnostic value of detecting soluble endoglin and soluble fms-like tyrosine kinase receptor 1 in maternal plasma.To explore new biomarkers which can predict the disorder.To compare the concentration of circulating angiogenic factors in maternal plasma in preeclampsia with it in normal pregnancy.To help doctors better understand the condition and identify the most severe potential cases early.METHODThe levels of soluble endoglin and soluble fms-like tyrosine kinase receptor 1 in serum were detected by enzyme linked immunosorbent assay.The study included all 43 women who had preeclampsia,containing mild preeclampsia(10 cases),early-onset severe preeclampsia(18 cases)and late-onset severe preeclampsia(13 cases),as well as 40 randomly selected controls,according to the diagnostic code on the textbook《obstetrics and gynecology》(the sixth edition)and cooper' s study.Maternal antecubital venous blood 3ml was collected into test tubes for the isolation of plasma.The ELISA method was used to detect the concentration of the angiogenic factors.Determine the optical density,using a microplate reader set to 450 nm.Calculate the average absorbance values for each set of reference standards,control,and samples.Construct a standard curve by plotting the mean absorbance obtaine for each reference standard against its concentration,and estimate the assay range by calculating the coefficient of variation of each standard constructing five independent standard curves.Useing the mean absorbance value for each sample,determine the corresponding concentration of it from the standard curve.Parametric statistics were used for data analysis.RESULTS1.The concentration of sEng from plasma samples:1)The levels of sEng in serum of the preeclampsia group(4.97±1.11)ng/mh was significantly higher than that of the controls[(1.26±0.40)ng/mL, P＜0.01].2)The levels were significantly higher in severe preeclampsia group than in mild group[(5.28±1.05)ng/mL VS.(3.94±0.53)ng/mL,P＜0.01].3)The level of sEng in serum of the EOSP group(5.81±1.15)ng/mL were significantly increased,as compared with(4.63±0.36)ng/mL in LOSP group(P= 0.001,P＜0.01).2.The concentration of sFlt-1 from plasma samples:1)The level of sFlt-1 in serum of the preeclampsia group(363.00±176.49) pg/mL was significantly higher than that of the control group[(89.11±27.57)pg/mL,P＜0.01].2)There were no remarkable difference in severe preeclampsia group than in mild group[(390.20±178.40)pg/mL VS.(273.25±143.30)pg/mL,P =0.066,P＞0.05].3)The level of sFlt-1 in serum of the EOSP group(430.45±220.88)pg/mL, as compared with(341.89±94.44)pg/mL in LOSP group(P= 0.356,P＞ 0.05),were all square.3.correlation analysis:There was no statistical significance(P＞0.05)between sEng and sFlt-1 as the coefficient correlation is -0.168.CONCLUSIONThe levels of soluble endoglin and soluble fms-like tyrosine kinase receptor 1 in serum of the preeclampsia group were increased significantly than that of the controls.More severe cases of preeclampsia had even higher levels of sEng,and had been positive correlated with hypertension and proteinuria.As biomarkers,sFlt-1 and sEng could help identify preeclampsia early,especially the most severe EOSP.