Screening The Effective Part Of Holly Bark For Bacteriostasis Anti-inflammatory And Analgesia Effect

Jiubiying is the Ilex rotunda Thunb drying tree bark or root bark of Holly Family,and its taste is bitter,and its nature is cold,and it can clear away the heat-evil and expell superficial evils and eliminate swell and alleviate pain,damp elimination,dispell wind-evil and neutralize poison,its synonym is Aquilaria sinensis(Lour.)Gilg,it record in the book of culling traditional Chinese medicine in the south mountain at first,which it is a civilian commonly used herb medicine in the south of china.The tree bark or root bark of jiubiying mainly contain flavonoid glycoside Phenols tannin and triterpene glycoside.The jiubiying alcohol extract have the effect of cutting down coronary artery flow stepping down heart rate weakening cardiac muscle contraction force raising the ability of bearing oxygen deficiency standing up to arrhythmia,in clinic it can cure heart brain blood vessel disease.But jiubiying decoction depress inaurate staphylococci haemolyticus chain coccus Bacterium flexneri Bacillus typhi aeruginosus Bacillus,it can cure much infectious diseases.Research objective:Existing research about Jiubiying mostly concentrates in the cardiovascular pharmacological action,but,regarding the effect of the anti-inflammation,the bacteriostasis,the analgesia pharmacology research is actually few.This research adopts the classics the system solvent extraction parting method,gets differently effect part of Jiubiying supposed to withdraw the spot,carries on the anti-inflammation,the bacteriostasis, the analgesia Pharmacology experiment,screens effective extraction spot.Research method:First separate: System solvent extraction separation:With the different polarity organic solvent,the benzine petroleum,the normal butyl alcohol,the absolute ethyl alcohol,the distilled water extract jiubiying,obtains the polarity from small to big extraction spot.Bacteriostasis experiment:Uses the test tube dilution method,by the golden yellow staphylococcus,the second grade hemolytic chain coccus,the white rosary fungus,carries on the smallest bacteriostasis density experiment to each different polar extraction spot to screen.Anti-inflammation experiment:Regarding in the bacteriostasis experiment the effect remarkable extraction spot,carries on the xylene induction mouse ear swelling,the lint ball induces the big mouse granuloma the anti-inflammation experiment.Analgesia experiment:Regarding in the bacteriostasis experiment the effect remarkable extraction spot,carries on the mouse hot plate experiment.Second screen:Regarding in bacteriostasis,anti-inflammation,analgesia experiment effective extraction spot,by silica gel column chromatographic analysis,with different polar elution solution elution separation,absolute ethyl alcohol group:Methyl alcohol:Chloroform(1:4),methyl alcohol: Chloroform(1:1),methyl alcohol:The chloroform(4:1)carries on the elution for the eluent,obtains 3 components,respectively isⅢ-1,Ⅲ-2,Ⅲ-3; Distilled water group:90%th,70%,50%,30%ethyl alcohol carry on the elution, obtains 4 components,respectively isⅣ-1,Ⅳ-2,Ⅳ-3,Ⅳ-4.Further the bacteriostasis experiment screens.Results:1,The benzine petroleum extraction spot of jiubiying have no effect to the golden yellow staphylococcus,the second grade hemolytic chain coccus, the white rosary fungus.The normal butyl alcohol extraction spot of jiubiying to the golden yellow staphylococcus,the second grade hemolytic chain coccus, the white rosary fungus have the small inhibitory action.The ethyl alcohol extraction spot and the distilled water extraction spot of jiubiying to the golden yellow staphylococcus,the second grade hemolytic chain coccus has the strong inhibitory action,to the white rosary fungus non-inhibitory action, or only has the weak inhibitory action.2,The ethyl alcohol extraction spot of jiubiying xylene induction mouse ear swelling to have the obvious inhibitory action(P＜0.05);The distilled water extraction spot of jiubying suppress the xylene induction mouse ear swelling not obviously to affect(P＞0.05)3,The ethyl alcohol extraction spot to induce the big mouse granuloma to the lint ball to have the obvious inhibitory action(P＜0.05),but low high monitoring team not obvious quantity effect relations.4,Jiubiying in the ethyl alcohol extraction spot hot plate mouse to have the obvious analgesia to affect(P＜0.05),but low high monitoring team not obvious quantity effect relations.5,Jiubiying in the ethyl alcohol extraction spot separation componentⅢ-1 pair of golden yellow staphylococcus,second grade hemolytic chain coccus inhibitory action;ComponentⅢ-2 has the strong inhibitory action;ComponentⅢ-3 inhibitory action is weak.Rescues must be supposed the distilled water extraction spot separation componentⅣ-1st,Ⅳ-2nd,Ⅳ-5 pair of golden yellow staphylococcus,the second grade hemolytic chain coccus have the strong inhibitory action,Ⅳ-3rd,Ⅳ-4 inhibitory action.Research conclusion:Jiubiying in the ethyl alcohol extraction spot to have the strong bacteriostasis,the anti-inflammation,the analgesia function, the distilled water extraction spot has the strong bacteriostasis function, but does not have the anti-inflammation and the analgesia function.After silica gel column chromatographic analysis elution separation,the bacteriostasis spot mainly occurs in the ethyl alcohol extraction spot methyl alcohol:Chloroform(1:1)elution part,distilled water extraction spot 90% ethyl alcohol and 70%ethyl alcohol elution part.