Influnec Of CO₂ Pneumoperitoneum To Biological Behavior Of Cervical Cancer Cell And To Respiration Ang Circulation

The laparoscopic technique has widely used in clinical practice now. Laparoscopic operation have many advantages over the tradition operation in good effect:less pain after operation;quick recovery and so on.so it has gradually become the important operation type for Benign gynecological diseases and has good prospect in Treatment of malignat tumor.But the application of laparoscopic technique have faced some new problems,such as: port site metastasis and intro-abdominal metastasis,the effects of CO₂ pneumoperitoneum on the organism,which have become hot issues.In this dissertation we study the effects of CO₂ pneumoperitoneum during laparoscopic operation on the biological behavior of cervical carcinoma cell and the organism with cervical cancer.We expect to preliminarily evaluate the safety of treatment of malignat gynecological tumors and provide clinical and laboratory evidence.The effects of CO₂ pneumoperitoneum to biological behavior of cervical cancer cell in vitroObjective:To investigate the effects of CO₂ pneumoperitoneum on growth,infiltration and metastasis of cervical cancer cell in vitro. Methods:With an artificial pneumoperitoneum model in vitrowere cervical cancer cells exposed to CO₂ insufflation of different pressure and different time,then we determine the cell growth curve,cell cycle and the adhesive,invasive ability.To observe the change of growth,invasion and metastasis of cancer cell.Experiment results:Proliferation after being exposed to CO₂ pneumoperitoneum can be inhibit and then accelerate,but is not related to the pressure of CO₂.the invasive,adhesive and invasive ability of cervical cancer cells after being exposed to CO₂ pneumoperitoneum decrease notably.Conclusion:CO₂ pneumoperitoneum can inhibit and then accelerate the proliferation,and inhibit the invasive,adhesive and invasive ability of cervical cancer cell.The effects of CO₂ to cervical cancer cell of growth and metastasis in VivoObjective:To investigate the effects of CO₂ pneumoperitoneum on growth and metastasis of cervical cancer cell in Vivo.Methods:To establish the nude mouse model of cervical cancer cell for CO₂ pneumoperitoneum.The mice were randomly divided into three groups(CO₂ pneumoperitoneum group,open abdomen group and Control group). Execute all the mice after four weeks,open the abdomen,search the organ in abdomen,disassociate the tumor tissue.Counting singulorum organ metastasis numbers,weigh metastasis tumor,detect the mice body weight degression after operation. Experiment results:the tumor weight increased obviously in CO₂ pneumoperitoneum group compare to open abdomen group and Control group.It had no predominance change in open abdomen group and Control group of tumor weight.The metastasis tumors were bigger on abdominal wall cut and more serious of growth and disseminateion in CO₂ pneumoperitoneum group. The mice weight descenting in open abdomen group was larger than CO₂ pneumoperitoneum group and Control group.Conclusion:CO₂ pneumoperitoneum can promote the growth and disseminateion of abdominal tumor than open abdomen operation.