The Mechanism Of Long-term Effects Of Physical Exercise On Neonatal Seizure-induced Cognitive Deficit

Objective To explore the long-term effects of neonatal seizures and physical exercise on learning and memory capacity。Methods The rats were randomly divided into the recurrent-seizure group (RS) and the control group. The volatile agent flurothyl was used to induce 30 min seizure attack. At postnatal day 6(P6), the recurrent seizures were induced once per day for consecutive 6 days. Control rats were placed into the container for an equal amount of time to their counterpart without exposure to flurothyl. At P29～P35,P61～P67, Y-maze test were performed to evaluate learning and memory capacity. During this time ,at P51～P56, the RS and contorl groups were submitted to an aerobic exercise program 30min per day for consecutive 6 days. At last, we detected the expresstion of GluR2 in hippocampus .Results Y-maze discrimination learning test: In the first Y-maze test, there was significant difference between the trials to criterion in RS group and control group(sP<0.05); but there was no significant difference between RS and control groups in the second Y-maze test..Memory test: The trials to criterion between RS group and control groups was not significantly different(P>0.05). There was significant difference expresstion of GluR2 between the RS group and control groups. Conclusions While neonatal seizures had long-term effect on spatial learning and memory, physical training can partly reverse it. The effect of single prolonged seizure on learning and memory need further investigation. Neonatal seizures could decrease the expresstion of GluR2 in dentate gyrus and Hilus of hippocampus.