| Objective: To study the expression of FHIT and Survivin protein in NSCLC and the correlation between them. Discussing their relationships to the biological behavior of NSCLC and the likely moleculemechanism,to supply an experiment basis for introducing FHIT, Survivin as new markmolecules into the clinical treatment of the tumors.Methods: An immunohistochemical method (PV-9000 Polymer Detection System for Immuno-Histological Staining) was used to detect the expressions of FHIT, Survivin proteins in 90 cases of lung cancer, 20 cases of adjacent tumor tissues and 10 cases of benign pulmonary disease tissues. SPSS14.0 statistic software was applied to analyze the data. P=0.05 was considered as level of tests. P< 0.05 was the standard of obvious difference.Results: 1.The positive rate (47.78%) of FHIT in lung cancers was significantly lower (P<0.01) than that in adjacent tumor tissues (90.00%) and benign tissues (100.00%). There was significant difference (P<0.05) between the positive expression of FHIT in squamous cancer (33.33%) and adenocarcinoma (64.29%). The positive rate of FHIT has relationship with the squamous cancer differentation level and lymph node metastasis (P<0.05). There was significant difference among the groups of clinical stage for FHIT expression (P<0.05). The positive expression rate of FHIT in non-smoking group is higher than that in smoking group (P<0.05). And it has no relationship with age, gender, tumor size, site ,adenocarcinoma differentation level and lymph node metastasis (P >0.05).2.The positive rate (65.56%) of Survivin in lung cancers was significantly higher (P <0.01) than that in adjacent tumor tissues (10.00%) and benign tissues (0). The positive rate was related to clinical stage(P<0.05), it was 52.94%,60.87%,81.82% in stageâ… , stageâ…¡and stageâ…¢respectively; and the positive rate associated with lymph node metastasis (P <0.05); but it has no relationship with age ,gender, tumor size, site, differation, pathological types and smoking(P >0.05). 3.Survival analysis employing Kaplan-Meier method indicated five-year survival rate in FHIT-positive subgroup (53.80%) was greater than that in FHIT-negative subgroup (14.11%) (Log rank test,x2 =16.465 , P=0.000);and five-year survival rate in survivin-negative subgroup(43.41%) was greater than that in survivin-positive subgroup (35.90%) (Log rank test, x2 =5.488 , P=0.019).4.The correlation was found between the expression rate of FHIT protein and Survivin protein.Conclusion:1.There was low expression of FHIT in NSCLC, and the expression was closely related with TNM staging, squamous cancer's differentiation and lymph node metastasis. The low expression of FHIT may participate in lung carcinogenesis and the development of lung cancer. And the low expression of FHIT is related with smoking. FHIT may be the target gene of smoking with lung cancer. The expression of FHIT in squamous is lower in adenocarcinoma, that shows FHIT may be not the key gene in adenocarcinoma carcinogenesis.2.The expression of Survivin in NSCLC was higher than that in benign tissues and related to lymph node metastasis and TNM staging. Survivin might play a role in lung carcinogenesis and development.3.Up-regulation of Survivin expression and down-regulation of FHIT expression predicts poorer prognosis in NSCLC.4.Correlation exists between the expression of FHIT and that of Survivin in NSCLC. They may take part in the carcinogenesis and progression of NSCLC together in complementary or cooperative manner. FHIT, Survivin is the important marker to evaluate the clinical pathology features of NSCLC.FHIT, Survivin may become important indexes of forecasting the invasion, metastasis, prognosis of NSCLC, and provide important targets for treating and preventing NSCLC in gene level. |
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