The Detection Of Drug-resistant And PFGE Genotyping Of MRSA Isolated From BurnWound

Objective To explore the distribution of the mecA genes in Staphylococcus aureusand the molecular epidemiological profile of Methicillin-resistant Staphylococ -cus aureus (MRSA). Methods Antibiotic resistant of 23 strains were determined by standard disk diffusion tests. The mecA genes were detected by polymerase chain reaction (PCR) methods and cefoxitin disk diffusion. Pulsed-field gel electrophoresis (PFGE) was used to type the chromosome DNA of MRSA isolates. Results Amongst 23 isolates of S. aureus the methicillin resistance gene mecA was found in 16 strains. All of MRSA isolates were susceptible to Vancomycin. The resistance to rifampicin was 87%. Three different PFGE patterns (A-C) were found among 16 MRSA strains and most of MRSA were type A (13 strains). Conclusion Cefoxitin disk diffusion method can be used reliably in detecting and confirming MRSA. MRSA is multi-resistant to antibiotics. A sub-type MRSA outbreak occured at Burn unit.