Experimental Study On Rabbits' Full-Thickness Articular Cartilage Defects Repaieed By Cocultures Of Dermal Pluripotent Stem Cell Seeded Onto Silk Fibroin/Polylactic Acid Scaffold

Objective: To investigate the feasibility of the dermal pluripotent stem cells in the repair of cartilage defects, it is aimed to provide experimental basis in view of cartilage defects of expanding the repair of cartilage defects seed cells selection. dermal Pluripotent stem cells and Silk fibroin/polylactic acid scaffold were cultured simultaneously for a period of two weeks , then, they were implanted into full-thickness articular cartilage defect of rabbit. after 12 weeks, rehabilitation organizations will be scored and the effect of restoration will be evaluated by statistical analysis, which will provide experimental and theoretical basis for the clinical application of tissue engineering methods to repair articular cartilage defects . METHODS: Neonatal cyanotic blue rabbits are used, to obtain dermal tissue directly isolated through mechanical method and acquire cells next through enzymatic digestion, the growth characteristics of adherent adhesion of stem cells is used to obtain high cloned cells, and subcultured accordingly. The cell concentration of 1×10~6/ml is cultured with Silk fibroin/polylactic acid scaffold for one week, after which the obtained result is implanted into full-thickness articular cartilage defects of rabbit. with 30 bluish violet blue rabbit growing for about 3-5 months, 60 joints in total, are randomly divided into three groups, A: dermal pluripotent stem cell and silk fibroin/polylactic acid scaffold B: Silk fibroin/polylactic acid scaffold, C: control, 20 joints each group. air embolization kills rabbits after 12 weeks, restoration organization is extracted to stain by HE and toluidine blue .in accordance with the repairing result of cartilage defect, which is performed by gross and histologic score, and analysed by statistics, the comparison of score difference of each group is verified whether it has statistical significance. Results: gross and histological observation proves that dermal pluripotent stem cell and silk fibroin/polylactic acid scaffold repair of the group organizations bears smooth surface, featured with a transparent, integrates well with the surrounding cartilage and subchondral bone. Polylactic acid scaffold group have a tiny transparent cartilage, fiber cartilage repair accounted for much proportion. the surface of control group shows some defects, mainly featured with fibrous cartilage repair. After gross and histological observations statistics score analysis is performed. results show that in comparision with group A, group B and group C, the repair of group A is most optimal in light of group B and group C (P <0. 05), consequently, group B is better than group C (P <0. 05). Conclusion: dermal multipotent stem cells to repair articular cartilage defects show an evident result, normal articular cartilage structure can be restored, and is feasible to be used as the seed cells of tissue-engineered cartilage.