Epigenetics Regulation Of B Cells In Systemic Lupus Erythematosus

Object:To investigate whether alterations in epigenetic regulation occur in B cells in systemic lupus erythematosus (SLE).Methods:(1) PBMC (peripheral blood mononuclear cells) cells were isolated from the peripheral venous blood of SLE patients and healthy donors by density gradient centrifugation. B cells were isolated using CD19 micro beads and protocols provided by the manufacturer.(2) Genomic DNA, total RNA was isolated with DNA/RNA kits.(3) The B cell global DNA methylation level was detected with Methylamp? Global DNA Methylation Quantification Kit.(4) The expression of DNMTs, MBDS, SIRT1, SUV39H2 and CREBBP mRNA were investigated with quantitative real time PCR.Results:(1) The global DNA methylation level in B cells had no significant difference between SLE patients and normal controls(P>0.05)).(2) The mRNA expression level of DNMT1, DNMT3a, DNMT3b,MBD1, MBD2, MBD3, MBD4 and MECP-2 had no significant difference between SLE B cells and normal B cells (all P>0.05).(3) Compare with normal controls, the mRNA expression level of SUV39H2 was significantly decreased in SLE B cells(P=0.01), and the mRNA expression level of SIRT1 and CREBBP had no significantly difference between SLE B cells and normal B cells(P>0.05, P>0.05, respectively).Conclusion:The data obtained suggests that DNA methylation appear normal in B cells in SLE.