| Background: Atherosclerotic plaque is classified two types: stable and unstable plaque. The rupture of unstable plaque is the major reason to cause acute coronary syndrome (ACS) and death of CHD. It has great importance to search the risk factor that make the plaque unstable and rupture. Some studies have found that HSP-60 was correlated with the degree of atherosclerotic lesion, and the level of serum HSP-60 was elevated in ACS patients, but there is no conclusion about the relationship between HSP-60 and the stability of atherosclerotic plaque. To study the relationship of HSP-60 and the stability of atherosclerotic plaque, we established the rabbit atherosclerotic model, then dealed with rosuvasatin, we examined the change of the inflammatory index that affect the stability of atherosclerotic plaque.Method: Eighteen rabbits were randomly divided into three groups: control group(n=6), hyper-lipid diet group(n=6) and rosuvastatin group(n=6), animals in control group received normal diet, the other two groups received iliac endothelium denudation after 4 weeks hyper-lipid diet feeding, then the two groups received hyper-lipid diet and hyper-lipid diet with rosuvastatin respectively. Serum TC, TG, HDL-C, LDL-C, HSP-60, IL-6, ox-LDL, SOD, MDA were measured at different time point. The rabbits were killed at 10 weeks. Iliac arteries were isolated and paraffin-embedded sliices and immunohistochemical staining of MMP-9 and HSP-60 were performed. Positive expressed area of MMP-9 and HSP-60 were calculated with image analysis software, and the correlation of them was analyzed.Results:1. The serum TG, TC and LDL-C level in hyper-lipid group were elevated significantly compared with control group (p<0.01) , the serum TG, TC and LDL-C level in rosuvastatin group were decreased significantly compared with hyper-lipid group (p<0.01) , and the HDL-C level was elevated (p<0.05) .2. The serum HSP-60, IL-6 level in hyper-lipid group were elevated significantly compared with control group (p<0.01), the serum HSP-60, IL-6 level in rosuvastatin group were decreased significantly compared with hyper-lipid group (p<0.01) , the HSP-60 level was positive correlated with IL-6. 3. The serum ox-LDL, SOD, MDA level in hyper-lipid group were elevated significantly compared with control group (p<0.01) , the serum ox-LDL, MDA level in rosuvastatin group were decreased significantly compared with hyper-lipid group (p<0.01), and the SOD level was elevated significantly (p<0.01). The HSP-60 level was positive correlated with ox-LDL and MDA.4. Atherosclerosis-stenosis and unstable-plaque model was successful established in hyper-lipid group, plaque obtained from rosuvastatin group was slight and more stable.5. The positive expressed area of MMP-9 and HSP-60 of in plaque obtained from hyper-lipid group was much more larger than rosuvastatin group (p<0.01) , the positive expressed area of MMP-9 and HSP-60 was positive correlated (r_S=0.736, p<0.01) .Conclusion:1. The serum HSP-60, IL-6 level in atherosclerosis-stenosis and unstable-plaque model were elevated significantly, and the positive expressed area of MMP-9 in plaque was increased, these findings demonstrate that unstable of atherosclerotic plaque was correlated with inflammation.2. The serum ox-LDL, MDA level were elevated significantly in atherosclerosis model, this demonstrates that oxidative stress was correlated with atherosclerosis.3. The effect of rosuvastatin regulating blood fat is very strong, then exerts its anti-atherosclerosis effect.4. Rosuvastatin can reduced serum HSP-60, IL-6, ox-LDL, MDA level and positive expressed area of MMP-9 in plaque, and elevated SOD level, these findings demonstrate that statins may exert its anti-atherosclerosis and stabilizing the atherosclerotic plaque effect through the anti-inflammation and anti-oxidation effect.5. HSP-60 may promote the release of IL-6, then induces the expression of MMP-9 and influences the stability of atherosclerotic plaque. |
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