Effect Of Hydroxyethyl Starch 130/0.4 On Global Cerebral Ischemia_reperfusion BBB In Rats

Objective: To observe the effects of Hydroxyethyl Starch 130/0.4 on global cerebral ischemia/ reperfusion in rats and to investigate the effect of HES130/0.4 of the underlying mechanism.Methods: Experiment protocol: The experiments were performed using 48 male Sprague-Dawley rats (weight 200～250g).The animals were housed in individual cages and allowed free access to water. Anaesthesia was induced by an intraperitoneal injection of chloral hydrate (0.35 g/kg body weight), and the animals were premedicated with 0.1 mg of subcutaneously administered atropine. The SD rats were randomly divided into 3 groups (n=16 each).(S group) sham operation: The vertebrobasilar artery and bilateral common carotid arteries will not be occlused; (R group) Ischemia Reperfusion group: The SD rats were subjected to three-vein occlusion : the vertebrobasilar artery and bilateral common carotid arteries would be occlused for 10 min; (H group) HES130/0.4+ I/R group: The vertebrobasilar artery and bilateral common carotid arteries would be occlused for 10 min,then injecting HES130/0.4 Starch at dose of 5ml/kg and rate of 0.2ml/min. To detect the change of blood gas at three time points, the blood sample was taken from femoral artery, The first point was the time of 10 min before the brain ischemia, the second was the time of the brain ischemia, the third was the time of 10 min after the reperfusion. To detect the change of blood hematocrit, the blood sample was taken from femoral artery , The first point was the time of 10 min before the brain ischemia, the second was the time of 10 min after the reperfusion the third was the time of 10 min before executed of rats..The brain tissue samples were taken from rats at 12 hours after reperfusion. There are 6 rats every group by random were executed and anterior of the left brain were removed for microscopic examination of pathology and measurement of the expression of NF-κB. The right brain tissue were removed for measurement of the content of TNF-α. 5 rats every group by random were determined by dry-wet weight method.Another 5 rats every group were opened thorax in a hour with injection of EB physiological saline (4ml/kg) in femoral vein When the rats were reperfused for 12 hours. To perfuse physiological saline(P=110mmHg) until the liquid of destrorotation become colorless, then execute the rats and take the whole brain. To removed the cerebellum, pituitary and blood for to measure the content of EB in brain.Results: The change of blood gas and Hct is normal at each time point.Compared with S group, the expression of NF-κB was increased in R group,H group(P<0.01). Compared with H group, the expression of NF-κB of R group were increased(P<0.01).Compared with S group, the content of TNF-αwas increased in R group,H group(P<0.01). Compared with H group, the content of TNF-αof R group were increased(P<0.01).The brain water content in the H group is lower than in R group (P <0.05). The data did not appeared statistical difference between S group and H group (P>0.05).The content of EB in brain in the H group is lower than in R group (P <0.05). The data did not appeared statistical difference between S group and H group (P>0.05).Conclusion: HES130/0.4 can lighten ischemia-reperfusion BBB injury in rat , reduce brain water content. HES130/0.4 have cerebral protection, the underlying mechanism may concerned with decreased TNF-αand NF-κB expression, lighten inflammation injury.