Protective Role Of Shenfu Injection (SF) In Ischemic Reperfusion Injury Of Rat Liver Graft

Objective: To investigate the protective role of Shenfu injection (SF) in ischemic reperfusion injury of rat liver graft and its mechanism.Methods: Seventy-two male Sprague Dawley (SD) rats were randomly divided into SF group and control group, and used as donors and recipients of orthotopic liver transplantation. The operation was performed according to the two-cuff technique. 2 hours, 4 hours and 6 hours after liver transplantation, serum and hepatic tissue of recipients were taken for following assays: TNF-α(tumor necrosis factor), NF-κB (nuclear factor kappa B) and ICAM-1mRNA(intercellular adhesion molecule-1 mRNA).The other thirty-six male SD rats were divided into three groups according to different reoxidation time, and accepted orthotopic liver reperfusion. All the cells got into hypoxia-reoxidation model. 2 hours, 4 hours and 6 hours after reperfusion, the cells were taken for following assays: NF-κB and ICAM-1.Results: Serum TNF-αof rat liver graft in SF group were (576±60pg/ml,518±52pg/ml and 421±35pg/ml) respectively, lower than in control group: (831±65pg/ml,715±69pg/ml and 598±52pg/ml, P<0.05). Moreover, expressions of NF-κB and ICAM-1mRNA in hepatic tissue of SF group were obviously poorer than control group. 2 hours, 4 hours and 6 hours after reperfusion, ICAM-1 levels of SEC in SF group were (44±3.4 pg/ml, 68±3.2 pg/ml and 73±4.5 pg/ml), lower than in control group: (59±4.3 pg/ml, 98±6.5 pg/ml and 111±5.7 pg/ml, P<0.05). And the expressions of NF-κB in SEC of SF group were obviously poorer than control group.Conclusion: SF could be benefit for hepatic tissue and SEC during ischemic reperfusion injury in the liver transplantation, and improve long-term outcome.