Study On The Determination Of Flavonoid Components And Hypoglycemic Activity In Potentilla Chinensis Ser

Traditional Chinese medicine Potentilla chinensis Ser is the Rosaceae the Potentilla L plant's dry entire grass. It has the distribution in the national most districts, while mainly produces in Shandong, Liaoning and Anhui.Its chemical composition mainly contain flavonoids, triterpenes, steroid category, phenols and derivatives with Qingrejiedu, Liangxue Zhilishen such as effectiveness, for dysentery abdominal pain, more than Jiuli, bleeding hemorrhoids, sore swollen Carbuncle drugs. It is the only plant of Potentilla chinensis Ser which is included by the People's Republic of China pharmacopoeia(2005 editions).At present, because of its relative Potentilla food plants Fanbai grass (Potentilla discolor Bge.) morphological characteristics of plants are very similar, some people mix these two medicinal plants to treat type 2 diabetes. There are many of the reported Fanbai grass hypoglycemic pharmacological activity of, and hypoglycemic effect is very clear. According to the study of the pre-discussion group, Potentilla dishes from the isolated compounds Fanbai grass and isolated the compounds, basically the same results. Therefore, this paper was commissioned Potentilla hypoglycemic pharmacological activity of the screening.In this thesis, three compounds have been isolated from the ethyl acetate extract of Potentilla chinensis Ser by repeatedly chromatography on flash chromatography, Sephadex LH-20, normal and reversed-phase silica gel columns. On the basis of the chemical evidence and spectra analysis data, the structures of three compounds were apigenin, quercetin and potengriffioside A.To develop a HPLC method to determine the content of 3 flavonoids in Potentilla chinensis Ser. The linear ranges of quercetin, potengriffioside A and apigenin were 0.72～10.08μg/mL, 0.86～12.04μg/mL and 0.54～7.56μg/mL, respectively (r= 0.999 9), the average recovery is 95.37%, 96.87% and 97.66%, the RSD is 1.79%, 1.63% and 2.08%, respectively; the content of 3 flavonoids in the herb is 100μg/g, 323μg/g and 55 μg/g, respectively. The methods established for determing total flavonoids of Potentilla chinensis Ser would lay a foundation for the quality control of Potentilla chinensis Ser。We choosed main effective components quercetin, potengriffioside A and apigenin as standard substance and determined the quality of Potentilla chinensis Ser with RP-HPLC. We also reviewed the factors that influenced the extraction of the content of total flavonoids in Potentilla chinensis Ser by orthogonal test design, further established the optimum extracting and separating procedure of total flavones from Potentilla chinensis Ser.Preliminary pharmacodynamical test showed that the compounds isolated from Potentilla chinensis Ser with different methods had lowering blood glycose bioactivities on Alloxan induced diabetics rats, the water extract from Potentilla chinensis Ser was significant, has statistics significance.This thesis can help people to recognize the quality standard of Potentilla chinensisSer and also apply to the science research and development of Potentilla chinensis Ser.