The Gene Expression Patterns Of Peripheral Blood Mononuclear Cells In SLE Patients

Part1 Bioinformatic analysis of SAGE library of PBMCs in SLE patientsObjective Systemic lupus erythematosus (SLE) is a systemic autoimmune disease with involving multiple genes.Peripheral blood mononuclear cells (PBMCs) play pivotal roles in SLE pathogenesis. The aim of this study was to outline the gene expression patterns of PBMCs in SLE patients by using serial analysis of gene expression (SAGE) technology.Methods Following construction of SAGE library of PBMCs collected from 3 cases of family SLE patients, a large scale of tag sequencing was performed. The data extracted from sequencing files was analyzed with SAGE 2000 V 4.5 software. The top 20 expressed genes of SLE patients were uploaded to http://david.niaid.nih.gov/david/ease.htm and the functional annotation of genes was obtained. The differences among those expressed gene were analyzed by chi-square tests.Results A total of 1286 unique SAGE tags were identified from 1814 individual SAGE tags. Among the 1286 identified unique tags, 86.8% had single copy, and only 0.2% tags had more than 20 copies. 68.4% of the tags matched known expressed sequences, 41.1% of which matched to more than one known expressed sequences. And 31.6% of the tags had no match and could represent potentially novel genes. Three tags were found to express differentially in SAGE library of SLE patients.Conclusion This study draws a profile of gene expression patterns of PBMCs in patients with SLE. Comparison of SAGE database from PBMCs between normal individuals and SLE patients will benefit us to understand the pathogenesis of SLE. Part 2 Differential expression of Neurogranin mRNA in SAGE library of PBMCs in SLE patients identified by RT-PCRObjective To investigate Neurogranin mRNA expression on PBMCs in patients with SLE Methods We analyzed the SAGE library of SLE based on the bioinformation, mRNA expression level of Neurogranin was measured with semi - quantitative reverse transcription- polymerase chain reaction (RT- PCR) of 20 patients with SLE and 15 controls. The relationship of the expression level of Neurogranin and SLEDAI scores was analyzed.Results The mRNA expression level of Neurogranin in active SLE was higher than those in both inactive SLE (P<0.05) and healthy controls (P<0.001), which was positive related to SLEDAI scores.Conclusion The mRNA expression level of Neurogranin increases in the SAGE library of SLE , which is identified by RT-PCR. Neurogranin may be involved in abnormal signal transconduction of immunocyte .