Expression And Significance Of Matrix Metalloproteinase-1 And Tissue Inhibitors Of Metalloproteinase-1 In Intestinal Adhesive Tissues

Objective: Through the big mouse intestinal adhesion animal model, examines MMP-1 and TIMP-1 in the intestinal adhesion organization and Normal tissue's expression, studies MMP-1 and TIMP-1 in the intestinal adhesion morbidity function.Method: Selects SD male gender big mouse 70, zhey were randomly allocated to three groups, Then take 10 rats as a normal group, the observation group was 30 rats with postoperative intestinal adhesion, the injured area drop dehydrated alcohol, the pliers clamps the blind arteria mesenterica to make the damage intestinal adhesion model. The control group 30 big mice and the model group same anaesthesias, operate the abdomen, but motionless intestines organization, direct pass abdomen. Separately after the postoperation 2d, 7d, 14d 3 phase takes 10 big mice respectively, they were sacrificed respectively. Take adhesive tissue of small intestine respectively, the expression of MMP-1 and TIMP-1 in the intestinal adhesive tissues and the normal intestinal tissues were determined by immunohistochemistry assay and image analysis.Results: 1, makes the mold technique latter 2 day of groups, MMP-1 and the TIMP-1 content exceptionally elevates, compares the difference with the normal control group to have the significance (P<0.01), MMP-1 climbs to the peak value. 2. makes the mold technique latter 7 day of groups, in the organization the MMP-1 content reduces; But the TIMP-1 content elevates to the peak value, compares the difference with the technique latter 2 day of groups to have the significance (P<0.01); Makes the mold technique latter 7 day of groups and the control group compares the difference also to have the significance (P<0.01). 3. makes the mold technique latter 14 day of groups, in the organization MMP-1 and TIMP-1 content drops, compares the difference with the technique latter 7 day of groups to have the significance (PO.01); Makes the mold technique latter 14 day of groups and the technique latter 2 day of groups compares the difference to have the significance (P<0.01); Makes the mold technique latter 14 day of groups and the control group compares the difference to have the significance (P<0.01).4. Each time point model group compared with the control group were significantly different (P <0.01).5. Each other in the control group showed no significant differenceT (P> 0.05).Conclusion: The MMP-1/ TIMP-1/ active change is one which of important adjustment factors in the intestines stick form.