| Objective:This study with biochemical detection research methods,Observed ascidian of experimental diabetic rats in vivo metabolism of oxygen free radicals,for the clinical application of scientific theory.Methods: Selection of 40 Wistar rats,males and females are both 20, Were divided into four groups in random:control group,the model group, ascidian group,glibenclamide group,In addition to the control group, other three groups are STZ model.The way of the model: One-time injection of peritoneal STZ 55mg/kg,The model of FBG>11.1mol/L are diabetes. Ascidian group gavages to the ascidian every day (Dose:20g/kg/d),Glibenclamide group gavage to glibenclamide every day (Dose:0.45mg/kg/d),the control group and control group gavage to saline every day (Dose:20ml/kg/d) ,Experimental cycle for four weeks.After 4 weeks,measured fasting blood glucose,glucose tolerance,with biochemical detection method measured nitric oxide(NO)in the blood,malondialdehyde(MDA), glycosylated serum protein(GSP)and the content of nitric oxide synthase(NOS),glutathione peroxidase(GSH-PX)and superoxide dismutase(SOD)activity;Detection of liver,kidney,pancreas tissue glycosylated serum protein(GSP)content.Results: The fasting blood sugar level of Ascidian group rats after the experiment was significantly lower than before the experiment ( P<0.01 ) ;SOD activity was significantly enhanced (P<0.01); GSH-PX activity was decreased, comparing with the control group P<0.05, comparing with the control group P>0.05;NOS activity was decreased (P<0.01);MDA was decreased (P<0.05); the content of NO was decreased (P<0.01); the content of GSP was decreased (P<0.01); the content of GSP of Liver, kidney, pancreas was decreased (P<0.01).Conclusion:1.The oral glucose tolerance of streptozotocin diabetic rats decreased significantly by oxidative stress.2.The function of sugar tolerance of streptozotocin diabetic rats were enhanced significantly by oxidative stress.3.The MDA, NO, GSP in blood and the GSP in the liver, kidney, pancreas of streptozotocin diabetic rats were significantly reduced by oxidative stress.4.The NOS, GSH-PX activity was significantly lower, SOD activity was significantly enhanced in blood of streptozotocin diabetic rats by oxidative stress. |
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