Study On Fingerprints Of Flos Sophorae Buds And Fructus Schisandrae Chinensis And The Interaction Between Its Effective Components And Serum Albumin

Traditional Chinese medicine fingerprint (TCM-FP) is one of the methods to evaluate the quality of TCM by specialization and similarity of fingerprint with the integrative overview. TCM-FP, as one of the effective means of quality control for TCM, has a great development in recent years. However, the actual state of TCM-FP emphasis on the chemical compounds of extracts and their relative contents, lack of the research on the curative effect of TCM. Based on the establishment of the fingerprint which is integrity, fuzziness and qualitified, the study established the functional fingerprint by introduce characteristic parameters, to make a great breakthrough in the concept and application of TCM-FP.The research projects and their results are:(1) The fingerprint spectrum of the Flos Sophorae Buds was established with methanol as the extraction solvent in high performance capillary electrophoresis with applied voltage of 20 kV and a buffer concentration of 40 mmol/L borate (pH 9.0). The fingerprint had good precision, stability and reproducibility. The RSD of relative migration time of the co-peak was below 1.3%. The RSD of the peak area above 10% of the total peak area was below 6.0%. These parameters meet the requirements for the setting-up of fingerprints of TCM. Took the different sources of Flos sophorae Buds (Guizhou, Jiangsu, Guangxi, Henan, Sichuan) as samples, the content of rutin was examined, and the similarity of their TCM-FP was evaluated by 3 different methods.(2) Rutin and quercetin, as the effective components of Flos Sophorae Buds, were selected to interact with bovine/human serum albumin. The four interaction systems were investigated by fluorescence spectroscopic method. The four interaction systems were also investigated by synchronous fluorescence spectroscopy.(3) The optimized electrophoretic electrolyte of microemulsion electrokinetic chromatography (MEEKC) was composed of a 50 mmol/L SDS, 80mmol/L n-heptane, 900 mmol/L n-butanol, 20 mmol/L borate solution (pH 9.0). Following the principle and method on the measurement of oil-water partition coefficients by MEEKC, a functional TCM-FP of Fructus Schisandrae Chinensis was established. The fingerprint had good precision, stability and reproducibility. The values of logPow of 13 components of Fructus Schisandrae Chinensis were examined by this method.