Research On The Antagonistic Effects Of CoQ₁₀ On Mouse Fibroblasts Injury Caused By UVB Radiation

BackgroundThe skin aging is classified into endogenous and exogenous aging, endogenous skin aging is an inevitable,natural aging process whose specific mechanism remain unclear.Among the exogenous factors for skin aging, ultraviolet radiation is predominant one.Skin aging due to the repeated exposure to ultraviolet radiation is called photoaging.UV radiation falls in nonionizing radiation,which produce different effects on cells.Based on the different wavelengths,ultraviolet wavelengths can be categorized into UVA,UVB and UVC.The biological effects of UVB is 800 to 1,000 times of UVA,which produces damage directly to DNA,induces oxidation reaction,causes reactive oxygen species(ROS) production,mediates inflammation and immune reaction,is the main spectrum for skin damageFibroblast is the dominant cells among dermis,also the main target of ultraviolet radiation.Research has demonstrated that fibroblast has played an important role in natural aging and photoaging of skin due to its biologica features.At present,ROS hypothesis is one of mechanisms of skin photoaging due to ultraviolet radiation.Antioxidants for skin may delay the occurrence of skin photoaging.There are already much research on vitamin C and vitamin E for skin antioxidants.Coenzyme Q₁₀ has been mainly used for the assistant therapy of acute and chronic congestive heart failure,viral hepatitis and cancer.There are few reports on the antagonistic effects CoQ₁₀ against skin photoaging caused by ultraviolet radiation.Mouse fibroblasts(NIH-3T3) were exposed to different doses of UVB radiation to explore the protective effects of CoQ₁₀ on fibroblasts. Malondialdehyde(MDA) content,the activities of superoxide dismutase(SOD), catalase(CAT),and glutathione peroxidase(GSH-Px) were to be determined to explore the antagonistic effects of CoQ₁₀ against ultraviolet radiation.Methods1.Mouse fibroblast cell line(NIH3T3) were grouped as following,0mJ/cm², 10mJ/cm²,30mJ/cm²,60mJ/cm²,90mJ/cm² of UVB irradiation.MTT assay was used to test the cell survival to establish UVB radiation cell damage model.2.Mouse fibroblast cells were to be seeded in 96 well plate and set up as control group,UVB injury group,protective group.24 hours after UVB radiation,the cells were to be collected from each group to determine MDA,SOD, CAT and GSH-Px by reagent kits.3.Data were to be analysed by SPSS 10.0 statistical software with the one-way ANOVA.Results1.With the increasing of UVB radiation dose,the mouse fibroblast survival rate decreased significantly(P＜0.05).The cell survival rate of UVB30mJ/cm² irradiation group decreased significantly compared with that of control group (P＜0.05).2.MDA level in UVB damage group was significantly higher than that in control group(P＜0.05);MDA level in protective group was significant lower compared with that in UVB group(P＜0.05).3.The activities of SOD,GSH-Px,CAT in UVB damage group were significantly lower than those in control group(P＜0.05);The activities of SOD,GSH-Px,CAT in the protective group were significantly higher than those in UVB injury group (P＜0.05).ConclusionCoQ₁₀ can produce antagonistic effects in mouse fibroblasts against UVB radiation.