| Objective: Primary liver cancer(PLC) is one of the commonest malignant diseases in our country. The mortality rate is the second of all diseases. Because of onset occult, it is often no obvious symptoms in the early period, most of them lose the opportunity of surgical resection when detected. So most patients receive transcatheter arterial chemoembolization (TACE), percutaneous ethano injection(PEI), radio frequency- ablation(RFA), focused ultrasound(FUS) or Chinese traditional medicine(TCM). According to the theory of TCM and clinical study over ten years, professor YaoShukun had developed Qinggan Huayu recipe with the therapeutic principles of clearing away heat and toxic material, removing blood stasis and resolving lumps, strengthening the spleen and replenishing qi. Researches had proved that Qinggan Huayu recipe had obvious repressive effect on the genesis of PLC. The mechanisms of oncogene, antioncogene, anti-angiogenesis, apoptosis, cell cycle and immune function on Qinggan Huayu recipe had been investigated which showed the mult-targets and integral regulation of TCM. This research was to observe the effect of Qinggan Huayu Recipe on primary liver cancer patients and the inhibition of drug serum of liver cancer patients treated by acetone or ethanol on hepatoma cells.Methods: According to a prognostic and clinical pathological criterion which mended by Society of Liver Cancer(CACA) in the 2001 Chinese liver cancer conference, 20 PLC patients were chosen , who had damp -heat of liver and gall with blood stasis syndrome pathological stageⅡb orⅢ(no faraway organ metastasis, liver function rank Child-Pugh B)PLC patients which had lost an operation chance and transcatheter arterial chemoembolization, all the patients company with varying degrees of abdominal pain,dyspepsia,jaundice,emaciation,hypodynamia ect. There were 13 males and 7 females, at the age between 46-75 years old, the average age was 53.2±5.5,Ⅱb patients with nine cases,Ⅲwere 11 cases.1 20ml Qinggan Huayu Recipe was taken by Patients, twice perday, aftermeals. 42 days was one treatment course. Anything raw, cold, greasy or spicy was avoid ate while taking medicatio(during treatment). The patients' clinical manifestation was observed and recorded everyweek, liver function and alpha-fetoprotein was checked every two weeks, CT was reexaminationed after use, the remission of tumor was observed .2 10ml venous blood of the patients were collected on weeks 0, 2, 4, 6 of the treatment, 2 hours after drug taken. The serum was joined four times acetone, well mixing, centrifuged at 3000r/min for the supernatants and then acetone was evaporated by warm bath as pretreatment. Tetrazolium bromide colorimetric assay(MTT) was taken to evaluate the inhibition effect of medicated serum pretreated by acetone on proliferation of SMMC-7721.3 10ml venous blood of the patients were collected on weeks 0, 2, 4, 6 of the treatment, 2 hours after drug taken. The serum was joined four times ethanol, well mixing, centrifuged at 3000r/min for the supernatants and then ethanol was evaporated by warm bath as pretreatment. Tetrazolium bromide colorimetric assay(MTT) was taken to evaluate the inhibition effect of medicated serum pretreated by ethanol on proliferation of HepG2.Result:1 The single points of abdominal distension,liver pain,dyspepsia,inappetence could be obviously reduced than those pre-administration. The respectively effective rate was 70.59%;76.92%;84.62%;83.33%. The average time for symptoms disappeared was 17.4,19.5,14.8,15.2, and not appear again after ease. The biochemical indicators and AFP were obviously decreased than those pre-administration, and by statistical analysis of each index in therapy mean have obvious difference. 0 was obtained complete relief by taking Qinggan Huayu Recipe, 0 was obtained partial relief, 12 cases stable, the stable rate was 60%. KPS score was improved in 15 cases, accounting for 75%, stabilized in 3 cases, lowed in 2 cases.2 Strong inhibitory effect was showed on multiplication of hepatocellular carcinoma cell which incubated for 24 hours with the Qinggan Huayu Recipe serum no-acetone-treated from PLC patients taking drug for 6 weeks. The 24 hours'inhibition rate was 28.6%,P=0.032.The 48 hours'inhibition rate was more obvious , up to 34.5%,P=0.035. A little weaker inhibitory effect was showed on multiplication of hepatocellular carcinoma cell incubated for 24 hours with the Qinggan Huayu Recipe serum acetone-treated from PLC patients taking drug for 6 weeks,which was 26.8% , P=0.029, compared with the no-acetone-treated serum of PLC patients, was up to 33.1%,P=0.024 for the 48 hours'inhibition rate. Parallel with the increasing time of patients'drug taking and incubation of hepatocellular carcinoma cell, the inhibitory effect was increased and was time-dependent.3 Strong inhibitory effect was showed on multiplication of hepatocellular carcinoma cell incubated for 24 hours with the Qinggan Huayu Recipe serum no-ethanol-treated from PLC patients taking drug for 6 weeks. The 24 hours'inhibition rate was 29.2%,P=0.043. More obvious was the 48 hours'inhibition rate,up to 36.2%,P=0.029. A little weaker inhibitory effect was showed on multiplication of hepatocellular carcinoma cell incubated for 24 hours with the Qinggan Huayu Recipe serum ethanol-treated from PLC patients taking drug for 6 weeks, which was 24.9%, P=0.022, compared with the no-ethanol-treated serum of PLC patients, and was up to 33.2%,P=0.028 for the 48 hours'inhibition rate. Parallel with the increasing time of patients'drug taking and incubation of hepatocellular carcinoma cell, the inhibitory effect was increased and was time-dependent.Conclusions: The clinical symptoms in patients with primary liver cancer could be significantly alleviated by taken Qinggan Huayu Recipe, the biochemical indexes and AFP could be reduced, liver function grade could be improved, and the tumor stable rate was 60%.The inhibition to SMMC-7721 of drug serum of PLC which was treated by acetone was weaker than that without acetone treated drug serum. It was demonstrated that after treated by acetone the interference of non-drug factors on the experimental was removed, then the pharmacology role of Chinese medicine was highlighting. However, it was probably that after treatment, the drug effects was arised after the endogenous inactivation was deactivated and then its original efficacy was reduced.The inhibition to HepG2 of drug serum of PLC which was treated by ethanol was weaker than that without ethanol treated drug serum.It was probably that after treatment, the drug effects was arised after the endogenous inactivation was deactivated and then its original efficacy was reduced . |
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