Study On The Anti-arrhythmia Effective Substances And Serum Pharmacochemistry Of Valeriana Officinalis L.

Valeriana officinalis L.was a herbal traditional Chinese medicine belongs to the family of Valerianace which was long used to treat illness such as disquiet,insomnia and aches of various kind and it has been written into .Modern research shows that,it has a great potential capability of curing cardiac arrhythmia.We separated the medicinal materials into oil,ethyl acetate extract,n-BuOH extract,water- extract. Pharmacological method was adopted to compare effect of different extracts.The result shows that the water-extract and oil have obvious anti-arrhythmia effect induced by trichloromethane and the water-extract and the n-BuOH extract have obvious anti-arrhythmia effect induced by aconitine. Then we merged the n-BuOH-extract and water-extract together and separated it with diffirent consistency of ethanol on the Macfofeticulf resin.The 90%-ethyl part shows good anti -arrhythmia effect induced by trichloromethane,so we defines it as the most effective part.The methods of solvent and chromatography were used to get 11 monomeric compounds from the 90%-ethyl part,and 8 of them were elucidated by spectroscopic spectrum as Chlorogenic acid(Ⅰ),Caffeic acid(Ⅱ),prinsepiol-4-0-β-D-glucoside (Ⅲ),(+)8-Hydroxypinoresinol-4'-0-β-D-glucopyranoside (Ⅳ),(+)8-Hydroxypinoresinol-4-0-β-D-glucopyranoside (Ⅴ),8-Hydroxypinoresinol-4-0-β-D-glucoside(Ⅵ),(+)-8-Hydroxypinoresinol(Ⅶ),Kaempferol(Ⅷ).The anti-arrhythmia effect of the 8 compounds is still screening.The Headspace Solid Phase Micro-extraction and Gas Chromatography(HS-SPME-GC) technology was adopted to analyse the composition of Valeriana officinalis L.HS-SPME-GC method gives us a new analytic method which shows a good capability of composition-extraction,Gas Chromatography also has good credibility and stability.The best condition of HS-SPME-GC was adopted in the Serum Pharmacochemistry study of different extracts and their serum sample.There is little similaries between the extract and the serum sample,so that the metabolized products may be effective substances.The research provided certain scientific parameter of anti-arrhythmia effective substances of Valerian.