Study On The Drug Effect Substances And The Mechanism Of Arrhythmic Effects Of Valeriana Officinalis L.

Valeriana officinalis L.belongs to department of Patrinia of valerian,its roots and rhizomes are the main parts of medicine which have sedative and spasmolytic effects, attending uneasy,palpitation insomnia,mania,rheumatism Bitongkang,dysmenorrheal,etc.Modern pharmacological studies have shown that valerian has anti-arrhythmic effect.In order to clarify the material basis of the anti-arrhythmic effects of Valeriana we adopted interdisciplinary research methods based on the previous studies.Through supercritical CO₂ extraction method and solvent method the Valeriana was divided into different valerian extracts,and then using aconitine alkali and chloroform to induce arrhythmia model for pharmacological screening indicators to determine the main active anti-arrhythmic site of Valeriana.The water-soluble parts of Valerian stably decrease the incidence of ventricular fibrillation,postpone arrhythmias time and reduce the mortality.Supercritical CO₂ extract and water-liquid parts have more pronounced antagonism to chloroform-induced ventricular fibrillation in mice.And the other parts also have anti-arrhythmic effects indifferent extent.Although inducing arrhythmia through different mechanisms,the water-soluble part of Valerian have altogether obvious antagonistic effects to the two agent chloroform and aconitine induced arrhythmia, while the other parts have different response.The all above reflect the mechanisms for its anti-arrhythmic is multifaceted and there are multiple active ingredients.Using chromatographic separation and spectral identification method,we can separate and identify five kinds of compounds from the effective anti-arrhythmic parts of Valeriana as follows:Green barbed tip lignan glycosides (prinsepiol-4-0-β-D-glucoside);8-hydroxy resin glycosides (8-hydroxypinoresinol -4-o-β-D-glucoside);β-sitosterol; kanokoside A;Chlorogenic acid.Using modern electrophysiological patch-clamp technique to investigate these compounds separated above,there are two compounds that have been proved had anti-arrhythmic activity: Green barbed tip lignan glycosides and 8-hydroxy resin glycosides.We explored the action mechanism of the green lignan glycosides and the results show that the Green barbed tip lignan glycosides through inhibiting potassium channels and prolonging effective refractory period to achieve the anti-arrhythmic therapeutic purposes.Green barbed tip lignan glycosides are expected to become anti-arrhythmic drug.The paper initially explain the mechanism of its anti-arrhythmic. On the basis of basic research of anti-arrhythmic substances we utilized high-performance liquid chromatography to research the fingerprint of water-soluble parts of Valeriana and demarcate 15 common peaks.Valeriana from different original Sources,different harvesting time and Valeriana close related species were also compared,establishing a reliable analytical method in accordance with the "Chinese Pharmacopoeia" and the State Food and Drug Administration issued technical standards.Thereby the research provides a reference for the Valeriana medicinal plants harvesting sources and season.Using high-performance liquid chromatography to analyze active anti-arrhythmic ingredients Green barbed tip lignan glycosides of Valeriana content has high sensitivity,good reproducibility,powerful reliability,through which we can effectively control Valeriana medicinal quality and provide a scientific basis to ensure drug safety.