Preliminary Study Of Membrane Progesterone Receptor Expression In Endometrial Carcinoma Cell Line

ObjectiveTo explore the expression of membrane progesterone receptor in endometrial carcinoma,verify the presentation of nongenomic action in endometrial carcinoma.Method1.Treat endomtrial carcinoma cell line Ishikawa by non-permeable progesterone-BSA conjugate labeled with fluorescein isothiocyanate(P-BSA-FITC),observe the location of P-BSA-FITC in the cell by fluorescence microscopy.2.The percentage of labled cells after different concentration of P-BSA-FITC was counted by flow cytometry.3.Detect p-ERK protein expression in endomtrial carcinoma cell line Ishikawa by western blot after treated by different concentration of P-BSA-FITC.Result1.Fluorescence on the membrane was visualized in cells treated with P-BSA-FITC. When cells were treated with BSA-FITC,no Fluorescence was seen under fluorescence microscopy.2.Cells showed higher percentage of fluence-labling in the P-BSA-FITC group than in the controled group,with the highest fluence-labling in the 25ug/ml P-BSA-FITC group.3.ERK expression was inhibited by P-BSA-FITC.The inhibition of ERK reached maximal level at 5min after stimulation with P-BSA-FITC of 25ug/ml.Conclusion1.There are progesterone receptors on the membrane of endometrial carcinoma cell line Ishikawa.The ability of membrane receptor to bind to progesterone is time and dose relateted.2.Progesterone had non-genomic action which was attibuted to the effect of progesterone membrane receptor by inhibiting the MAPK signal transduction pathway.3.Both genomic and non-genomic action of progesterone are invovled in the control of endometrial carcinoma.