The Study On The Effects Of Fructus Schisandrae Chinensis Freeze-Dried Powder On Improving Sleep Using Drosophila Melanogaster As The Model Organism

As a basic behavior and an important physiological phenomenon in human, sleep quality has a significant effect on people's life and health. Scientists have confirmed that not only sleep can maintain the vitality of the body, allay tiredness, recover spirit, but also improve the work and learning efficiency. For this reason, more and more people have drawn attention to the problem of sleep quality. However, along with the rapid rhythm of modern social living, the exaggeration of the work pressure, Insomnia has been a common disease and the motivation of some other diseases.The adjusting process of sleep is very complicated and the mechanism of sleep remains unknown in spite of decades of research. Although many interesting hypotheses have been proposed, none have been comprehensive enough to gain general acceptance. Recently, scientists have found many features of sleep in drosophila are similar to human's sleep, such as behavioral, pharmacological, molecular and genetic approaches. So, drosophila with well-established genetics had become an ideal model organism for sleep research.In our studies, we used an Infrared Drosophila Activity Monitor System (DAMS; Trikinetics Inc. USA) to record sleep state of drosophila. We observed and analyzed the recording data for continuous 24 hr, and defined sleep time that drosophila's quiescing time is exceed 5 min. We investigated the affect of sex and age on total sleep time and sleep-wake cycle in drosophila with index of sleep time by analysis of data. Then we studied the best delivery factors of Fructus Schisandrae Chinensis Freeze-dried Powder in improving drosophila sleep and taked the 7-day-old drosophila as the objects of observation according to the climb activity. At last, we verified the effect of Fructus Schisandrae Chinensis Freeze-dried Powder on improving sleep of insomnia drosophila caused by two different ways of sleep deprivation—illumination and mechanical stimulation. First, our results indicated: through observing the gram of sleep-wake cycle, the young fruit flies have a long sleep time and a good continuity. With growing, the sleep of drosophila was fragmented and has similar features to human's sleep.Second, nyctohemeral activity of drosophila is frequent in both light-dark transitional period and dark-light transitional period(7 hr and 19 hr) and show two activity peaks. With the increasing in age , two activity peaks were more and more unobvious. There are some difference between the male and female. The sleep of the female mainly happen at night, but the male mainly at noon and night. The male have a longer sleep time than the female.Third, 8% drug substratum for dose, 6 days for delivery time is the best to the female(p<0.01); 8% drug substratum for dose, 4 days for delivery time is the best to female(p<0.01).Last, Fructus Schisandrae Chinensis Freeze-dried Powder has an effect on insomnia drosophila induced by illumination or mechanical stimulation.