The Expression Of Nuclear Factor-kappa B In Epithelium Of Human Anterior Lens Capsule With Age-related Cataract

Objective To explore the role of nuclear transcript factor kappa B(NF-kB)on the occurrence and development of the age-related cataract by examining the different expression of NF-kB in epithelium of human anterior lens capsule between the group with age-related cataract and the control group.Methods 60 anterior capsules with age-related cataract(case group,A group)and l0normal lens(normal control group,B group)were collected by microscope.The expression of NF-kB P65 protein in 30 anterior capsules with age-related cataracts and 5 normal lens were detected respectively by immunohistochemistry and analyzed by color graphic-based analysis system(OLYMPUS PRO150ES).The expression of NF-kB P65 mRNA in 30 anterior capsules with age-related cataracts and 5 normal lens were detected respectively by real-time polymerase chain reaction(real-time PCR).And their differences were compared and treated using Independent-Samples T test in statistical packages SPSS11.5.Results The yellow granules can be seen in epithelial cell cytoplasm and nucleus of age-related cataracts and normal lens.Light yellow granules mainly be seen in the cytoplasm of anterior lens capsule with normal lens and brown granules mainly be seen in the nucleus of anterior lens capsule with age-related cataract.Compared with normal control group,the expression of NF-kB p65 protein significantly increased in case groups. Significance is P＜0.05 based on Independent-Samples T test(t=6.2109).Compared with normal control group,the expression of NF-kB p65 mRNA significantly increased in case groups.Significance is P＜0.05 based on Independent-Samples T test(t=12.9683).Conclusion NF-kB may be a kind of transcript factor to maintain the elementary metabolic function in lens epithelial cells.The over-expression of NF-kB may play an important role on the occurrence and development of the age-related cataract.