The Study Of The Effect Of Low-Intensity He-Ne Laser On The Red Blood Cells Of α-Light Thalassemia Using Laser Tweezers Raman Spectroscopy

Objective: To make use of laser tweezers Raman spectroscopy system, Study the differences of red blood cells between normal human and patients withα-light thalassemia, and to discuss the effects and mechanism of low-intensity He-Ne laser on red blood cells from normal andα-thalassemia patients .Methods: To take the fresh blood samples from healthy donors andα- light thalassemia patients,using MR450-type low-intensity He-Ne laser vertically irradiated red blood cell suspension by different power (2mW～5mW) and different irradiated time (40s, 10min, 30min, 1h) . To obtain Raman spectra of red blood cells by laser tweezers Raman spectroscopy system.Results: 1.Comparred with normal RBC (red blood cells), overall intensity of Raman spectroscopy of RBC inα-light thalassemia is weak; Some characteristic peaks have happened a few displacement: spectrum of 1212 cm-1, 1224cm-1 integrated as one a feature of the peak 1216cm-1. Shows that there must some spectral difference of RBC betweenα-ight thalassemia and normal. 2. The effect of low-intensity He-Ne laser on normal RBC was not obvious in Raman spectra. 3. The effect of low-intensity He-Ne laser on RBC fromα-light thalassemia patients:⑴Comparred irradiated group with control group, the overall intensity of irradiated group is also weak; the relative intensity of 1622cm-1 and 1604cm-1 increased mainly because of the spectral 1604cm-1 intensity reduced; for spectral 1004cm-1,the intensity decreased and the peak shape sharped and FWHM reduced. (2) Different irradiated time has a different effect: an hour later after laser irradiation time 40 seconds,the intensity of 754cm-1 reached minimum; an hour later after irradiated 10min ,the relative intensity of the spectrum 1622cm-1 and 1604cm-1 significant reduced; and same effect has seen at 30min later after irradiation 30min and immediately after irradiation at 1h.Conclusion: 1.Laser tweezers Raman spectroscopy provides useful information in the difference of red blood cells between normal human and patients withα-light thalassemia. 2.The appropriate power and the appropriate irradiated time of low-intensity laser play important part in the oxygen-carrying function of RBC fromα-light thalassemia patients, and also impacted cell structure. These effects after the cessation of laser irradiation for a certain period is still maintained, and may reached maxmoium, this indicates that the delayed effect and the cumulative effect of low-intensity laser on theα-light thalassemia red blood cells is more obvious.