The Study Of Red Blood Cells In Three Common Cardiovascular Diseases Using Optical Tweezers Raman Spectroscopy

Objective: Making use of optical tweezers Raman spectroscopy system to study whether there are differences among normal human red blood cells, umbilical cord blood RBC and the RBC in patients with common heart disease and to investigate the changes of the contents in RBC and its variation mechanism, so as to provide valuable information for the diagnosis of disease. Methods: Taking the fresh venous blood samples from healthy blood donors, patients with common heart disease and umbilical cord blood of fetal which is delivered but the placenta is not peeled, and then isolating RBC after washed and centrifuged, and determining Raman spectra of RBC by optical tweezers Raman spectroscopy system.Results:1. The differences of RBC Raman spectra between umbilical cord blood and normal human are not obvious. 2. Compared with umbilical cord blood RBC, the overall intensity of Raman spectra in patients with congenital heart disease is significantly different. The overall spectrum of RBC in patients with congenital heart disease is weaker then umbilical cord blood RBC. Some characteristic peaks have a few displacements. Spectrum of 1273 cm-1 moved to 1264 cm-1; Intensity of spectrum of 1546 cm-1 reduced significantly. 3. RBC Raman spectra of normal human and patients with other heart disease :(1) Comparison between normal humans'RBC and RBC in patients with arrhythmias:â‘ Some of the overall intensity from patients with arrhythmias is also weak.â‘¡The spectrum of 1453 cm-1 moved to 1449 cm-1.â‘¢The intensity of spectral of 863 cm-1 weakens and peak deformation widens the peaks of spectrum 1212 cm-1 and 1226 cm-1 combine into spectrum 1216 cm-1. In addition, the low intensity He-Ne laser irradiation on RBC in patients with arrhythmias has a certain impact. (2) Comparison the RBC between normal humans'and patients with myocardial infarction:â‘ There was only little change in the overall intensity of two spectrums.â‘¡The intensity of spectrum 1273 cm-1 reduced significantly.â‘¢The spectrum of 1078 cm-1 moved to 1089 cm-1.Conclusion:(1) Optical tweezers Raman spectroscopy can determine and analysis the difference of RBC between umbilical cord blood and patients with congenital heart disease and provide useful information for prenatal diagnosis, which may provide a new, quick and easy methods of spectral analysis for improving the detection rate of fetal congenital heart disease. (2) By comparing the Raman spectra of normal RBC and three kinds of RBC in patients with heart disease, we can understand the changes of RBC molecular structure, conformation and composition in different types of cardiovascular disease, and further study Raman spectra model of cardiovascular disease. We can also investigate the type and pathogenesis of cardiovascular disease from the molecular level and provide reliable experimental evidence for clinical diagnosis.