The Study Of The Correlation Between Serum Levels Of Chemokines MCP-1, PF4, IL-8 And Coronary Atherosclerotic Heart Disease

Objectives: Coronary Atherosclerotic Heart Disease (CHD) is a multifactorial process. Inflammation play an important role in the beginning and development of CHD. It is a critical step in inflammation that the activation and recruitment of mononuclear cells into the vessel wall. This step happens not only at the formation of the plaque, but also at the disruption of the plaque and the thrombokinesis. The family of chemokines is a series of cytokines that have the resemble structure and function.The molecular mass is about 8~12KD. Chemokines can be devided to four subgroup of CXC, CC, C and CX3C according to the location and function of the residual of aminothiopropionie acid. It is chemokine regulates the recruitment and infiltration of mononuclear cells. Three groups were enrolled in this study: control group, stable angina pectoris group and acute coronary syndrome group (including unstable angina pectoris group and acute myocardial infarction group).Through testing serum levels of Monocyte Chemoattractant Protein-1, Platelet Factor 4 and Interleukin-8:1 explore the relationship between expressing of monocyte chemoattractant protein-1(MCP-1), platelet factor 4(PF4), Interleukin 8 and forming coronary atheromatous plaques .2 explore the connection of monocyte chemoattractant protein-1(MCP-1), platelet factor 4(PF4), Interleukin 8 and stability of coronary plaques.3 investigate the character of serum levels′variation of MCP-1, PF4 and IL-8 on different time in ACS patients.Methods: We selected subjects who were in cardiac department in TangShan worker′s hospital during September 2007 to June 2008. According to the patients′coronary artery angiogram results and clinical symptom, we divided 150 people into 35 control subjects, 35 SAP patients and 80 acute coronary syndrome patients (including 40 UAP patients and 40 AMI patients). People that who has the disease of organ transplantation, infection of HIV, kidney disease, other inflammations, tumors, wounded or operated within two weeks, serious liver or renal dysfunction, cerebral vascular accident within one month, disease of immune system and treated with immunomodulating drugs, taken medicine of Statin within one week before hospitalization were eliminated. Serum levels of MCP-1, PF4 and IL-8 were determined by ELASA. Statistical evaluation was performed with SPSS13.0 software. Before statistical evaluation, the normality and the homogeneity of variance of statistical data should be tested. Data were expressed as Mean±SD. Differences between two groups were analyzed by T-test. Differences among more than two groups were analyzed by one-way ANOVA and SNK-q test. Two-side P<0.05 was considered statistical difference.Results: 1 Compared with control subjects, the serum levels of MCP-1, PF4 and IL-8 elevated obviously in CHD patients(including SAP and ACS group)(P<0.01).2 The serum levels in ACS patients was much higher than SAP patients(P<0.01).3 The character of serum levels′variation of MCP-1, PF4 and IL-8 on 1-hour after entering into hospital, 48-hour after PCI and 1 week after PCI in ACS patients was dissimilar, and the levels of 48-hour after PCI was highest (P<0.01).Conclusions: 1The serum levels significantly increased in CHD patients, it implicated that they might take part in the actions of informing atheromatous plaques.2 The serum levels in ACS patients was much higher than SAP patients, it demonstrated that they may develop the unstability ,disruption of the plaque and the thrombokinesis.3 The result that serum levels of MCP-1, PF4 and IL-8 were dissimilar on different time provided a proof of anti inflammation therapy for us.