| Background and ObjectiveObesity and insulin resistance (IR) are associated with elevated risk of atherosclerosis disease. Compared with equally obese insulin-sensitive patients, those obese patients with IR suffered from increased risk for coronary heart disease (CHD). Change of cytokine levels may promote the occurrence of IR. Inflammatory reaction could occur at various stages of the atherosclerotic which from the beginning of lipid stripes to the late of complex lesions. Proinflammatory cytokines may promote coronary plaque fibrous cap to get thinning and plaque-rupturing, followed by thrombosis, and lead to the occurrence of CHD. Therefore, it is of great significance to study the relationship between cytokine expression levels and CHD.Omentin is a adipocytokine, and is highly expressed in visceral omental adipose tissue. The gene expression and plasma levels of omentin in obese and overweight population were significantly lower than that in the lean population, low level of omentin was related to the degree of obesity and IR. Omentin has been shown to participate in the inflammatory response of the body. In patients with chronic inflammatory bowel disease, omentin mRNA expression levels were different. The omentin concentrations of synovial fluid in patients with rheumatoid arthritis decreased. From above-mentioned, it’s suggested that omentin was correlated with obisity, IR and inflammatory response. Omentin-1is the main circulating form of omentin in plasma, and the study data related to omentin-1in patients with CHD was little. Interleukin-18(IL-18) is an inflammatory cytokine, and its level increased in obese human serum. It’s found that IL-18is related to IR. Serum IL-18levels were related to body mass index (BMI), IR index in patients with polycystic ovary syndrome with IR. IL-18was originally known as gamma interferon (IFN-γ)-inducing factor, IFN-γ is a proinflammatory cytokine, which can activate macrophages and a variety of other cells, and is involved in the antherosclerotic plaque-rupturing process. One study found that serum IL-18levels increased significantly in patients with CHD compared with that in the control group, which suggesting that IL-18is one of the important markers of reflecting inflammatory response of coronary disease.Interleukin-16(IL-16) is a chemotactic factor of CD4+cells. IL-16could enhance antigen-stimulated peripheral blood mononuclear cells to secrete IFN-γ, studies have shown that IFN-γ could promote the formation of atherosclerosis. Under the role of IL-16, monocytes and mature macrophages could secrete interleukin-1β, interleukin-6, TNF-α and interleukin-15, and these cytokines were closely related to the occurrence and development of atherosclerosis or (and) CHD. IL-16may participate in the formation and development of atherosclerosis through impacting on secretion of various inflammatory mediators. Recently it was reported in the literature that in Chinese Han population, IL-16rs11556218G/T gene polymorphism was significantly related to the risk for CHD. So far, there wasn’t report about the relationship between plasma IL-16levels and CHD.In this study, we would explore the relationship of plasm omentin-1, IL-18and IL-16levels with CHD through detecting these cytokines levels of patients with CHD and patients in the control group.Part ⅠThe study of the relationship between plasma omentin-1, interleukin-18levels and coronary heart diseaseObjectiveTo investigate the relationship between plasma omentin-1, interleukin-18(IL-18) levels and the pathogenesis of coronary heart disease (CHD) through detecting plasma omentin-1and IL-18levels of patients with CHD and patients in the control group.Methods87patients hospitalized from May2011to November2011were divided into two groups66cases in the coronary heart disease group (38cases in the unstable angina pectoris group,28cases in the stable angina pectoris group), and21cases in the control group. The height, body mass and blood pressure of all the patients were measured, and electrocardiograph (ECG) was graphed. Fasting venous blood of each of selected candidates was extracted to assay fasting glucose, total cholesterol, triglyceride, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol. Coronary angiography was conducted in a week after hospitalized, Gensini score rating was used to evaluate the extent of coronary stenosis. The enzyme-linked immunosorbent assay (ELISA) was used to detect plasma omentin-1and IL-18levels.Results1. The plasma omentin-1level in CHD was lower than that in the control group, and the difference between the two groups was statistically significant (p<0.01). The plasma IL-18level, Gensini score in CHD group was higher than that in the control group, and the difference between the two groups was statistically significant (p<0.01), while the omentin-1, IL-18level and Gensini score in the UAP group and SAP group were not statistically significant (P>0.05).2. Omentin-1was negatively correlated with BMI (r=-0.016,.P<0.01), while IL-18was positively correlated with BMI (r=0.154, P<0.01).3. IL-18was positively correlated with Gensini score (r=0.092,P<0.01).4. Systolic blood pressure, FG, TC, HDL-c, LDL-c, omentin-1, IL-18and Gensini score were correlated with CHD (P<0.05or P<0.01).5. Omentin-1and IL-18level were correlated with CHD respectively (P<0.05or P<0.01).ConclusionsPlasma omentin-1levels decrease and IL-18levels increase in patients with CHD, and omentin-1and IL-18are associated with CHD, which suggesting that omentin-1 and IL-18play a role in the pathogenesis and development of CHD, and this provides new ideas for intervening pathogenesis and development of CHD. Part ⅡThe study of the relationship between plasma interleukin-16levels and coronary heart diseaseObjectiveTo investigate the relationship between plasma interleukin-16(IL-16) levels and the pathogenesis of coronary heart disease (CHD) through detecting plasma IL-16levels of patients with CHD and patients in the control group.Methods87patients hospitalized from May2011to November2011were divided into two groups66cases in the coronary heart disease group (38cases in the unstable angina pectoris group,28cases in the stable angina pectoris group), and21cases in the control group. The height, body mass and blood pressure of all the patients were measured, and ECG was graphed. Fasting venous blood of each of selected candidates was extracted to assay fasting glucose, total cholesterol, triglyceride, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol. Coronary angiography was conducted in a week after hospitalized, Gensini score rating was used to evaluate the extent of coronary stenosis. The enzyme-linked immunosorbent assay (ELISA) was used to detect plasma IL-16level.Results1. The plasma IL-16level in CHD group was higher than that in the control group (p=0.01), while there was no significant difference between the IL-16level in the UAP group and SAP group (p>0.05). Gensini score in CHD group was higher than that in the control group, the difference was statistically significant (p<0.01).2. Systolic blood pressure, FG, TC, HDL-C, LDL-c, IL-16and Gensini score were correlated with CHD (p<0.05orp<0.01).3. IL-16was correlated with CHD (p<0.05).ConclusionsPlasma IL-16levels increase in patients with CHD, and IL-16level is associated with CHD, which suggesting that IL-16may play a role in the pathogenesis of CHD, and this will provide new target for intervening the pathogenesis of CHD. Objective To investigate the relationship between plasma omentin-1, interleukin-18(IL-18) levels and the pathogenesis of coronary heart disease through detecting plasma omentin-1and IL-18levels of patients with coronary heart disease and patients in the control group. Methods87patients (without diabetes mellitus) hospitalized from May2011to November2011were divided into two groups66cases in the coronary heart disease group (38cases in the unstable angina pectoris group,28cases in the stable angina pectoris group), and21cases in the control group. Venous blood samples were collected from the patients after an overnight fast after admission to hospital, and electrocardiograph of each patient was traced, coronary angiography was conducted. The plasma omentin-1and IL-18levels were detected by enzyme-linked immunosorbent assay (ELISA), and the height, body mass, blood pressure, fasting blood sugar, total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol, Gensini score and other indicators were measured. Results The plasma omentin-1level in the coronary heart disease group was lower than that in the control group, and the difference between the two groups was statistically significant(P<0.01). The plasma IL-18level and the Gensini score in the coronary heart disease group were higher than that in the control group, and the difference between the two groups was statistically significant (P<0.01). The plasma omentin-1level was negatively correlated with BMI (r=-0.016, p<0.01), IL-18level was positively correlated with BMI (r=0.154, p<0.01). The plasma IL-18level was positively correlated with Gensini score in the coronary heart disease (r=0.092,p<0.01). The plasma omentin-1level and the plasma IL-18level were correlated with coronary heart disease respectively. Conclusion The plasma omentin-1levels decrease and IL-18levels increase in patients with coronary heart disease, and the levels of these two cytokines are correlated with CHD respectively, which suggesting that these two cytokines may play a role in the pathogenesis and development of the coronary heart disease. Objective To investigate the relationship between plasma interleukin-16(IL-16) levels and the pathogenesis of coronary heart disease through detecting plasma IL-16levels of patients with coronary heart disease and patients in the control group. Methods87patients (without diabetes mellitus) hospitalized from May2011to November2011were divided into two groups66cases in the coronary heart disease group(38cases in the unstable angina pectoris group,28cases in the stable angina pectoris group), and21cases in the control group. Venous blood samples were collected from the patients after an overnight fast after admission to hospital, and electrocardiograph of each patient was traced, coronary angiography was conducted. The plasma IL-16levels were detected by enzyme-linked immunosorbent assay(ELISA), and the height, body mass, blood pressure, fasting blood sugar, total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol, Gensini score and other indicators were measured. Results The plasma IL-16level in the coronary heart disease group was higher than that in the control group, and the difference between the two groups was statistically significant (p=0.01). The Gensini score in the coronary heart disease group was higher than that in the control group, and the difference between the two groups was statistically significant (P<0.01). The plasma IL-16level was correlated with coronary heart disease. Conclusion Plasma IL-16levels increase in patients with CHD, and IL-16level is associated with CHD, which suggesting that IL-16may play a role in the pathogenesis of CHD, and this will provide new target for intervening the pathogenesis of CHD. |
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