Penehyclidine Hydrochloride In The Rat Model Of Acute Paraquat Intoxication Induced Lung Injury And Its Impact On On Of MMP-9 And MPO

Objective To observe the effects of Penehyclidine Hydrochloride on expression of MMP-9 and MPO in the rat model of acute paraquat intoxication induced lung injury and to study the effects of Penehyclidine Hydrochloride on acute paraquat intoxication induced lung injury and the mechanism. Whether Penehyclidine Hydrochloride can reduce expression of MMP-9 and MPO in lung injure induced by acute paraquat intoxication.Methods Divide sixty healthy Sprage-Dawley into three groups randomly: (i) Acute paraquat intoxication group (group P, 25mg/kg i.g. n=20). (ii)Penehyclidine Hydrochloride therapy group (group T, 0.5mg/kg i.p. n=20) and (iii) Control group (group C, n=20).At 3,6,12,24hours after exposure, the level of MMP-9 and the activities of MPO in lung tissue of the rats were detected respectively, and the extravascular 1ung water volume was determined. HE staining was also performed to detect the injury of lung tissues.Resultsâ‘ C ompared with that in group C, MMP-9 activities and expression of MPO in lung tissue of group P markedly increased (P <0.05) except at 3 hours group. â‘¡T here was a significant decrease in MMP-9 activities and expression of MPO in the lung tissues in Penehyclidine Hydrochloride-treatment group comparing with Poisoned group(P<0.05).â‘¢Compared with that in group C, the extravascular lung water volume was decreased significantly in the Penehyclidine Hydrochloride therapy group of 12h and 24h subsets (P<0.01) , however it was more pronounced in Poisoned group (P<0.01).â‘£Compared with group C, inflammatory cell infiltration was increased markedly. Penehyclidine Hydrochloride can slow down the development of the progressive diffuse airsacculitis.Conclusion The obtained results showed:â‘ Expression of MMP-9 and MPO in lungs of rats increased significantly after acute paraquat intoxication.â‘¡Penehyclidine Hydrochloride may regulation the expression of MMP-9 and Leukocyte accumulation.â‘¢Penehyclidine Hydrochloride might have a therapeutically effect in preventing the pathological changes of pulmonary edema and the development of lung injury.