Research Of Effects Treated With Gubenpingchuantang Of ECP,MBP In Airway Of Guinea Pigs With Bronchial Asthma

【Objectives】At the base of good therapeutic effect of gubenpingchuantang in preventing and curing bronchial asthma.We did further research to observe therapeutic influence of gubenpingchuantang on animal model of asthma in some indicators such as ECP,MBP and EOS etc.In order to discuss mechanism of action of this compound on treatment of asthma and provide theoretical evidence for clinical application.【Methods】60 guinea pigs(Half male and female)weight 250-300g were randomly divided into normal control group(A group),model group(B group),western medicine control group(dexamethasone) group(C group),Chinese medicine treatment of low-dose group(01 group),Chinese high-dose treatment group(D2 group),12 in each group,we used the model Underwood made and and improved it.In the first day of experiment,each guinea pig in group B,C,D1,02 was given intraperitoneal injection of 10ml suspl,consists of OVA10mg,100mg dry powder aluminum hydroxide and inactivated pertussis vaccine 5×10~9mL to allergize,guinea pig in group was injected equal volume of normal saline for instead;In the 15th day we seted guinea pigs needed to be allergized in a 14 liters transparent glass Bell,and gave them 1%OVA ultrasonic atomizing inhalation(aerosol volume from small to big) about 15-20 seconds,then made them natural inhalation 30min,once every day,60min before excitation every guinea pig was given intraperitoneal injection of 10mg/kg of diphenhydramine to prevent allergic reactions which would cause death,guinea pigs in group A was ultrasonic atomized equal volume of normal saline for instead;The standard of successful asthma model is asthma-like performance emerge such as:head,facial pruritus,breathing deepen,less moving,camponotus,reduced forelimb lift,head to breathe,cough,nasal fan,convulsions and so on.From the Beginning of the 16th day guinea pigs in treatment groups were administration by gastric tube,guinea pigs group A and group B were feed with equal volume of normal saline,which in group C were feed with injection of dexamethasone(1mg/kg);which in group D1,D2 were feed with concentrated liquid of gubenpingchuantang(SunburnEphedra,Almond,Licorice,erillaseed, White mustard seed,Raphani,semen,Scutellaria,HERBA EPIMEDII etc.)(2g crude drug per ml) D1 group(2.5g / kg) and the D2 group (7.5g/kg) the treatment of administration last about 7 days.After the treatment,animals in every group were killed to collect BALF, carotid artery and lung tissue samples in order to observe the total number of BALF cells and quantity changes in various types of it, we observed morphology and pathological changes in lung tissue, used enzyme-linked immunosorbent assay test for determining content of serum ECP,we observed EOS after HE staining and did MBPmRNA RT-PCR semi-quantitative analysis of bronchial smooth muscle,we used statistical software of SPSS13.0 for for data statistical analysis;count data expressed with(?)±s,compared with test.measurement data used X~2 test for statistical analysis.【Results】Guinea pigs in group A were in good condition,no one was died.Sensitized guinea pigs were poor-spirited,less moving, food and water intake decreased significantly,and returned to normal in 1-2 days.In the 16th day,3 guinea pigs in group B,4 guinea pigs in group C,2 guinea pigs in group D1 emerge the phenomenon of fluffying fur.When the second Induced asthma,3 guinea pigs in group B died after a serious asthma attack.After treating by administration the situation of Guinea pigs improved, but it can not restore to the level of the group A,lung tissue blood vessels damage of guinea pigs in group D2 repaired,alveolar septal became narrow,epithelial hyperplasia improved and reduced inflammatory cells,alveolar space is clear.Compare With the group B,airway inflammatory cell infiltration was significantly reduced, just like group C;The total number of BALF cells,eosinophils and lymphocyte infiltration decreased,compared with group B there were significantly different(P＜0.01);The distribution of serum gcP in asthmatic guinea pigs were significantly decreased compared with the group B there were also significant differences(P＜0.05).so was MBPmRNA(P＜0.01).【Conclusions】Gubenpingchuantang can induce or promote reducing airway EOS of asthmatic guinea pigs which reduce the airway inflammatory cell infiltration and control of chronic airway inflammation,and achieve the purpose of prevention and treatment of asthma.Its mechanism are possibly by changing the expression of the ECP and MBP in lung tissue of asthmatic guinea pigs,and reduce content of ECP and MBP in lung tissue,and induce EOS apoptosis, so that infiltration of eosinophils cells in BALF of lung tissue significantly reducedand the number of lymphocytes is significantly reduced,which reached the purpose of prevention and treatment of asthma.