Protective Effect On The Model Of Rats' Intracranial Inflammation Induced By IL-8

Objective:To confirm the intracranial inflammation-induction activity by Interleukin-8(IL-8) and observe the protective effect of hypothermia.Methods:40 neonatal rats were divided randomly into four groups including saline injection group,IL-8 injection and hypothermia in early stage group,IL-8 injection and hypothermia in later stage group and IL-8 injection without hypothermia group.As described in the group, we injected IL-8 solution by intracranial acupuncture.After that every group of rats got hypothermia(33℃) treatment in 2h,8h or no hypothermia at all,respectively.Then NSE, S-100 protein and Ck-BB in cerebrospinal fluid from the rats of all the four groups were measured,just before the tissue from the rat brains were paraffin embedded for pathological and immunohistochemical examination.Result:The NSE,S-100 protein and Ck-BB level of the rats from saline group were obviously lower than those of the rats injected with IL-8(P＜0.01).The NSE,S-100 protein and Ck-BB level of the rats from IL-8 injection and hypothermia in early stage group were obviously lower than those of the rats receiving hypothermia later or those put in the normal circumstances(p＜0.01).The results of pathological and immunohistochemical examinations indicated that the IL-8 injected rats suffer more from inflammation than the rats from the saline group,judged by the density of neutrophlic granulocyte and the marking material of neutrophils:myeloperoxidase.Conclusion:IL-8 injection could induce severe intracranial inflammation.Hypothermia with good timing could inhibit the process of in??mation induced by IL-8 and thus take effect on the neuroprotection.