The Damage Of Mitochondra In The Chronic Alcoholic Myopathy

The chronic alcoholic myopath (CAM) is a kind of myopathy because of longtime alcoholic misuse.Their frequently clinical appearance was having difficulties in gait,various muscle symptoms such as cramps,local pain and reduced muscle mass.So,chronic alcoholic myopathy affects the quality of patients'life severely.It's research is more less than alcoholic liver disease,alcoholic cardiomyopathy and alcoholic peripheral nerve disease.Some research show that oxidation and anti-oxidant disbalance affect progress of CAM.It's important for ROS in the disbalance between oxidation and anti-oxidant.Mitochondra ia a important organelle .Its functions include production ROS except synthetic ATP. Mitochondra not only the organelle in which production ROS but also the target organ which ROS attract at.The literatare about mitochondria in the CAM is most about synthesis ATP presently in China and other countries. There is no repotr about the injuries of mitochondra in the CAM.It's important research the ralation between the injuries of mitochondra and the disbalance between oxidation and anti-oxidant. So our investigation that the alcoholie disease should be eoncentrate on the meehanism and the pathway of the damage that to the mitochondrium.Objective: By setting up rat modele of CAM,to determin the oxidation and anti-oxidation index,and to investigate the change of mitochondral membrane fluidity,the content of cyt-c,mitochondrial menbrane potential (△Ψm),which are the correlated indexes about the damage of mitochon- drion,to approach the effect and mechanism of CAM through mitochondria pathway,thus to reveal a new view of mechanism of chronic alcoholic myopathy and to provide experiment basis for clinical therapy. Methods: 60 health,male,Sprague-Dawley rats,were separated rando- mly into two groups(control and alcohol),treated on the basis of setting up the CAM rat myopathy model,at the same time,they are feeding the same improved food with high fats and sufficient nutrients,to observe the rat body weight and the change of praxiology.At the end of 12 weeks,rats were killed,takenthe muscle of posterior limb,after separating plantaris,to prepare the frozen section of the plantaris to be confirmed to form the myopathy model by the histochemical stain.Spectrophotometer was used to measure the activities of superoxide dismutase(SOD),Glutathione peroxi- dase(GSH-Px) and the content of malondialdehyde(MDA) in the muscle specimens of each group respectively. Fluorospectrophotometer was used to measure different groups of mitochondrial membrane fluidity; was used to detect the expression of cyt-c of each group and mitochondrial menbrane potential (△Ψm) was measured by flow cytometry(FCM).Results:1. The body weight(BW) of rats shows:it was significantly lower than the control group.2. Spectrophtometer method shows:MDA in alcoholic group were significantly higher than control group,activity of SOD and GSH were lower than control group.3. Fluorospectrophotometer shows: In the CAM, mitochondrial membrane fluidity descend.4. Western blot shows: the expression of cyt-c decrease in alcoholic group. 5. Flow cytometry shows: mitochondrial menbrane potential were lower in alcoholic group than control group.Conclusions:1. The increase of ROS relates to the damage of mitochondria in the CAM .In the CAM, mitochondrial membrane fluidity descend; mitochondrial menbrane potential were lower; the expression of cyt-c decrease.2. Mitochondrial membrane fluidity descend in the CAM show that the function of mitochondrial membrane protein was damaged.3. The expression of cyt-c decrease and mitochondrial menbrane potential were lower show that mitochondra were damaged. 02.- increase in CAM.