Study On The Central Nerve Depressant Effect Of The Iridoids Of Valeriana Jatamansi Jones

Valeriana jatamansi Jones which also named Matixiang,Laohuqi and Xiangcazi et al is the rhizoma of the plant Valeriana jatamansi Jones of Valeriana Linn of Valerianaceae. The earliest records is in the "Compendium of Materia Medica", 1977 edition of Chinese Pharmacopoeia(a)has contained it. It is warm in nature, acrid and bitter flavor. It has function of regulating qi to alleviate pain, to check diarrhea, expel wind-evil and remove wetness. Utended in abdominal distention, gas pains, dyspepsia, diarrhea, dysentery, rheumatic arthralgia and lassitude loin and legs. The modern chemistry study found its main had iridoid, sesquiterpenes, alkaloids and flavonoids four types of chemical composition.This topic mainly researchs about abstraction technology of Iridoids in Valeriana jatamansi Jones, the central inhibition of Iridoids, Iridoids effect to content of central neurotransmitter.Concrete substance as follows:1. Cuming the total count of iridoids as the investigative indicatrix, laying extraction method, perpendicular experiment design proposal and assay method of Iridoids in Valeriana jatamansi Jones. Using hypersound extraction and percolate extraction method, discern contriving perpendicular experiment L₉(3⁴), adopting electric titration determine the content of iridoids. From the result of hypersound extraction we can realize the best artwork is A1B3C3D3 , scilicet 95% density and 12 times alcohol, extraction time is 50 minutes, and extracting 3 vices. The content of iridoids in the extractive is 7.05%. From the result of percolate extraction we can realize the best artwork is A₃B₂C₂D₂ scilicet 65% density and 10 times alcohol, coarse powder, extraction time is 12 hours. The content of iridoids in the extractive is 7.63%. When considering a short ultrasonic extraction, high efficiency, easy operation, and thus determine the final selection process for the ultrasonic extraction A1B3C3D3.2. From the sedative and hypnotic, analgesic, anticonvulsant three aspect studied Iridoids component in Valeriana jatamansi Jones of it's central inhibition action. Study on the sedative and hypnotic effects through researching in mouse autonomic activities, subthreshold and limes doses continual hypnotize effect. The results showed that the Iridoids in Valeriana jatamansi Jones had the conspicuous effect of the mitigate and hypnosis. The results of relieve pain experiment showed that Iridoids in Valeriana jatamansi Jones had the inhibitory effect on intraperitoneal injection of acetate and foot pain caused by plantar subcutaneous injection of formalin, but to the heat-induced pain had no significant inhibitory effect. Experimental use of anticonvulsant pentylenetetrazol, strychnine, and isoniazid induced convulsions in mouse. Iridoids on three drug-induced convulsion that caused by the incidence of isoniazid-induced no significant effect on mortality, but reduced pentylenetetrazol, strychnine-induced mortality, and can be extended to three drug-induced convulsion convulsion latency.3. This topic using pre-column derivatization HPLC method research the mouse brain tissue composition onγ-aminobutyric acid (GABA), glutamate (Glu), glycine (Gly), aspartate ( Asp) four central neurotransmitter content by Iridoids. The experimental results showed that the three doses of iridoids are making GABA content increased (P<0.05,P<0.01), had a lower trend of Glu content, low-dose group were significantly maker Gly content cut down, had no significant effect on Asp content.From the above experiment can be seen Iridoids ingredients had sedative, hypnotic and the analgesic effect. The incubation period of convulsion mouse can be extended. Reduced pentylenetetrazol, strychnine-induced mortality. The central inhibition mechanism may relative with Iridoids can make rejection capability neurotransmitter GABA content increased, and make excitability neurotransmitter Gly content degrade.