Identification Of The Transcriptional Profile In Cigarette Smoke Condense-Induced Human Bronchial Epithelial Cells And Mechanism Of Alteration

Objective: To confirm the previous microarray results of cigarette smoke condense (CSC)-induced malignant alteration of transcriptional profile in papillomavirus-immortalized human bronchial epithelial cells (BEP2D) and explore the mechanism of CSC-induced malignant alteration in BEP2D cells.Methods: RT-PCR,Northern blot and Real-time PCR analysis were performed to identify differential expression genes alteration of transcriptional profile, and treated P40 of CSC-induced BEP2D cells with 5-Aza-CdR, then analyzed a batch of genes with RT-PCR.Results: The alterations of mRNA expression,which detected by some technologies, matched those detected in the microarray assays; compared with the control, the mRNA expression of some genes in the CSC treated P40 of BEP2D cells are increased after treated with different concentrations or days of 5-Aza-CdR.Conclusion: The alteration of genes expression profiles in the CSC-induced BEP2D cells was successfully analyzed by microarray technique, and CSC-induced hypermethylation of genes should involved in the process of malignant transformation in BEP2D cells.