The Study Of Reversal Effect Of Elemene On Multidrug-resistant Of Human Osteosarcoma Cell Line (MG-63/ADM) And The GST-Ï€ Relate To The Effect In Vitro

Objective:To study the Reversal Effect of Elemene on Multidrug-resistant of human osteosarcoma cell line(MG-63/ADM) in vitro and investigated the relationship between GST-Ï€and the effect.Methods:To investigate the IC₅₀ of adriamycin(ADM),IC₀₅ and IC₁₅ of elemene(ELE) to MG-63 human osteosarcoma cell by MTT assay. The MG-63 human osteosarcoma cell divide into three groups randomly (A,B,C Group).Group A is the control group,to establish a multi-drug resistant of human MG-63 cells by short-time pulse exposure of the cell to the IC₅₀ of adriamycin.The cell of Group B,C are exposed both in the same dosage of ADM with the control group and IC₀₅(group B) or IC₁₅ (group C)of elemene.The exposure continued until the cell of Group A can be cultured stably in the nutritive medium which contain IC₅₀ of ADM,then drug exposure are stopped in all experiment Groups.we repeat to investigate the IC₅₀ of adriamycin to all post-drug intervention MG-63 human osteosarcoma cells by MTT assay.And intracellular accumulation of ADM were determined by fluorospectrophotometery. Immunohistochemistry was applied to study the GST-Ï€expression of initial and post-drug intervention MG-63 cells.At the end,we analyse the experimental result by statistics. Results:â‘ The IC₅₀ of adriamycin(ADM) to MG-63 cells were 3.37μg/ml(GroupA),2.21μg/ml(GroupB),1.19μg/ml(GroupC),and the RF values were 11.62(GroupA),7.62(GroupB) and 4.10(GroupC),the comparisons among Group A,GroupB and Group C have statistical significance(P＜0.05).â‘¡The intracellular accumulation of ADM all experiment groups were 1.21μg/10⁶ cells(Group A),1.96μg/10⁶ cells(GroupB),2.71μg/10⁶ cells(Group C),Group A＜GroupB＜Group C,the comparisons among Group A,GroupB and Group C have statistical significance(P＜0.05).â‘¢The GST-Ï€expression rates of initial and post-drug intervention MG-63 cells were 10.25%(initial cells), 35.75%(Group A),25.25%(Group B) and 20.50%(Group C),the comparisons among Group A,GroupB and Group C have statistical significance(P＜0.05)Conclusion:Firstly,we conclude that the multi-drug resistance to ADM of human osteosacoma cell(MG-63/ADM) could be reversed by Elemene with non-toxic or low-toxic dosage;Secondly the expression of GST-Ï€could be regulated down by Elemene;The last,the increased intracellular accumulation of ADM and downregulation of GST-Ï€would contribute to the reversal mechanism of Elemene.