The Expression Of Serum Inhibin B In Patients With Varicocele And The Changes Of Serum Inhibin B Before And After Surgery

Objective:The Objective of this study was to examine the effect of varicocele treatment on the quality of spermatogenesis,as reflected by serum inhibinB levels,and to investigate the involvement of Sertoli cell-germ cell interaction in the pahtophysiological mechanism.Methods:Twenty nine consecutive patients present at XiangYa Hospital with varicocele who were complaining of infertility were included in the study. Varicocele was graded according to physical examination color-Doppler ultrasound.Eighteen gradeⅡand eleven gradeⅢpatients were included in this study.All patients were treated surgically by high spermatic vein ligation.Serum inhibinB,FSH,LH,T and sperm concentration were measured before and after operation.Ten unaffected adult male of age-matched were used as controls.Likewise Serum inhibinB,FSH,LH,T and sperm concentration were measured.And thenⅠcalculated their changes and correlation.Use enzyme linked innunosorbent assay(ABC-ELISA) testing serum inhibinB level.Use radioimmunoassay to test serum FSH,LH,T level.To compare the endocrine and sperm concentration,Paired-Samples T test was used. Bivariate Correlation was used to calculate correlations.And Independent-Samples T test was used when comparing the same indicator in two groups.Results:1.Before the varicocele operation,the serum inhibinB levels inⅡdegree patients is 153.69±20.19pg/ml,inⅢdegree patients is 142.68±29.68pg/ml,which are all higher than the serum inhibinB levels in the control group(P＜0.01 and P＜0.01).We can not observe significant difference of the serum inhibinB levels betweenⅡdegree patients andⅢdegree patients(P=0.242)2.In all of the varicocele patients receiving varicocele operation,a significant increase in serum inhibinB levels was observed from 149.51±24.22pg/ml pretreatment to 175.65±27.13pg/ml after treatment(P＜0.01).3.No significant changes were observed in serum levels of FSH and LH.A significant increase in serum T levels was observed from 445.66±95.94mIU/ml pretreatment to 464.68±97.54mIU/ml after treatment(P＜0.01).4.Semen analysis showed a significant improvement in sperm concentration,from 15.00±2.73×10~6 /ml pretreatment to 18.89±2.96×10~6/ml after treatment(P＜0.01).5.The serum inhibinB levels were inversely correlated with serum FSH levels both before and after treatment(r=-0.896,P＜0.01 and r=-0.758,P＜0.01). 6.The serum inhibinB levels were not significant correlated with serum FSH,LH and T levels.7.The serum inhibinB levels were positive correlated with sperm concentration both before and after treatment(r=0.937,P＜0.01 and r=0.899,P＜0.01).Conclusions:Serum inhibinB can be as a sensitive marker,which can evaluate the fertility of patients with varicocele,including information pre- and after operation.Serum inhibinB is a sensitive and accurate marker and is easy to operate,so it could be used widely in chinic.