Study On The Expression Of Transforming Growth Factorβ1 And Smad7 Protein In Lichen Planus

Objective:This study aim to evaluate expression of transforming growth factorβ1 and Smad7 protein in lichen planus,and investigate their possible role in pathogenesis of lichen planus.Methods:The immunohistochenmical 2-step was used to detect the expreesion of transforming growth factorβ1 and Smad7 protein with the transforming growth factorβ1 rabbit anti human polyclonal antibody and the Smad7 protein rabbit anti human polyclonal antibody in 30 cases of lichen planus and 25 cases of normal skin. Leica manual microscope and Qwin image analysis system(v2.3,Leica Inc.,German) were used to analysis the positive results with integral optical density,then measure the positive product with its integral optical density,then analyze the differences of transforming growth factorβ1 and Smad7 protein expressed in the experimental group and the control group by principle of statistics.Results:The study showed that the expreesion of transforming growth factorβ1 in normal skin was in majority basal cells of epidermis;while in lichen planus the expreesion of transforming growth factorβ1 was negative.The expreesion of transforming growth factorβ1 in lichen planus was lower than in normal skin(t' =7.380,P＜0.05).The Smad7-immunohistochemistry was seen significantly in diseased region of lichen planus,the coloring of epidermis was continuous or focus-form of full-thickness,the positive coloring was mostly in basal cells,acanthocyte and mainly granulosa cells,the expression of Smad7 protein in the infiltrative lymphocytes which were in the superficial layer of dermis was negative;while the expression of Smad7 protein in the epidermis and dermis of normal skin was negative.The difference of the two groups was significantly(t'=10.088, P＜0.05).Conclusion:The absence expression of transforming growth factorβ1 and the positive expression of Smad7 protein in lichen planus prompt that may act an certain role in the pathogenesis of lichen planus.The study result support that the pathogenesis of lichen planus was related to the immunologic abnormality.