The Effects Of Acupuncture On The Cathartic Colon Rats' Stools

Objective: We researched on the droppings of constipation rats with water drying, pH determination and qualitative add semi-quantitative bacterial culture methods to detect the moisture, pH, bacterial content of the cathartic colon rat droppings.Through inter-group cross-referencing and male and female control etc,we try to find out the effects of acupuncture on the cathartic colon rat droppings,and preliminary study on the mechanism and the effectiveness of acupuncture for the slow transit constipation (STC).Methods: 1.Grouping the animals: selecting 60 successful model SD rats, half male and female. Completely according with the random method of grouping and gender . Divided them into acupuncture group ,gender.drug group, with acupuncture and drug group, sham acupuncture group, model group.5 big groups 10 small groups.The split among the three groups of rats after the body weight, sex is no difference. SD of normal control group, 12 rats, 6 male and female each, divided into 2 groups by gender. 2.Set up the experimental model: model rats were Ig with the rhubarb powder suspension regular daily,the rhubarb powder has been through a 80 mesh sieve.The first dose is 150mg/kg·d,then progressively increase 150mg/kg·d, when 50% rats had diarrhea , maintain this dose to 80% rats' diluted stool disappeared.And then continued progressively increase 150mg/kg·d, when 50% rats had diarrhea,maintain this dose to 80% rats' diluted stool disappeared.Repeat the above steps again for two and a half months,when 80% rats' diluted stool disappeared for the third time, maintain the dose for one week,then cessated drug delivery and offer these rats the ordinary soft-feed.The experiment model was successfully made,so we can stop setting up model. 3.Specimen collection: collecting rat droppings when stopped gavaging and treatment for moisture analysis, pH analysis and bacterial culture. 4.Statistical treatment: applicate SPSS13.0 statistical software to analysis the data.Results:1.Normal SD rats Ig with the rhubarb powder suspension regular daily can be used to establish "cathartic colon" models,and these models can simulate virtual Peru (2 Deficiency of both Qi and Yin deficiency or yin and yang) with a number of indicators;2."Cathartic colon" rats:①fecal water content significantly decreased, by about 42 percent of normal water content dropped to 37 percent moisture content;②24 hours stool grains per kg body weight also significantly reduced, specifically, by the normal of around 63 down to about 47;③fecal pH was alkaline,by the normal of 6.69 increase to 6.87;④stool is in a dysbacteriosis situation .Saprophytic fecal bacteria (Escherichia coli, Enterococcus large) content increased, the corresponding probiotic (Bifidobacterium, Lactobacillus) content is lower than the normal state;3.When it comes to increasing the fecal moisture , the best method is acupuncture combined with medicine, the effects of acupuncture and drugs are equal.This indicates that STC patients with stool colorectal or induration on clinical should adopt the method of acupuncture combined with drugs;4.When it comes to increasing the 24 hours stool grains per kg body weight, the best method is also acupuncture combined with medicine,and the effects of acupuncture and drugs are also equal.This indicates that STC patients with less stool volume or less stool frequency in certain time should adopt the method of acupuncture combined with medicine;5.Fecal pH is slightly higher than normal when constipation, acupuncture and drug treatment are both efficacy and the effects are equal,other methods have no effects on it;6.The possibility of STC patients' self-healing when stop treatment is very small.But large intestinal enterococci have certain self-repair capacity.However,in these experimental groups, only simple acupuncture can effectively reduce the large Enterococcus content;7.Acupuncture and acupuncture combined with medicine can improve the E.coli of rats in constipation status, and their effects have no differences;8.In these experimental groups, only acupuncture combined with medicine can improve the bifidobacteria content of fecal when rats are in constipation status;9.In these experimental groups, only simple acupuncture can improve the lactobacillus content of fecal when rats are in constipation status;10.When it comes to gender, only the drug group has differences in fecal water content and 24 hours stool grains per kg body weight,and results show that female is better than male.The reason is probably that female and male rats have different drug resistance.It indicates that we should lower the dose when treat female rats;11.Generally consider all the observation indicators, and clinical medical expenses ,we recommends that: for STC patients, the propriate treatment method is simple acupuncture treatment; acupuncture combined with medicine is suitable for patients with hard stool or less qualitative; When patients' fecal flora analysis indicates dysbacteriosiscircumstances,they should try to avoid oral cisapride or drugs alike,but can use preparations which containing probiotics.