The Effects Of Probucol On Oxidative Stress In The Kidney Of Type2 Diabetic Rats

Objective: To establish the animal model of type 2diabetes mellitus and to observe the levels of nitrotyrosine and 8-hydroxy-2-deoxyguanosine in the kidney, which were the biomarkers of oxidative stress and renal function in diabetic rats by treated with probucol in early stage.Methods: 25 male Wister rats of 8weeks old randomly devided into C(n=7)and B(n=18).C was the normal control group ,feeding with regular diets ,4 weeks later injected citrate buffer liquid in abdominal cavity; B was the type 2 dibetes model group, feeding with the diets enriched with high-fat and high-sucrose, 4 weeks later injected the aqua of streptozotocin(STZ) at a dose of 35mg/Kg body weight in abdominal cavity; Then B was devided into D and N. N was the probucol group, feeding with the diets enriched with high-lipid, high-sucrose and prebucol. 8 weeks later , measured the body weight , renal weight, renal weight/body weight, 24h alb, the fasting plasma glucose (FPG), fasting insuline (FINS), MDA and SOD. To observe the expression of NT, 8-OHDG by immunohistochemistric method . Made the correlation analysis of MDA ,SOD , NT , 8-OHDG and ALB , Cr. Results: The FPG, 24hALB, BUN, Cr and MDA level of C and D group were significantly increased (P<0.05), and the average optical density of NT and 8-OHDG were much higher than C group (P<0.05) , but the markers of N group decreased significantly to compared with D group (P<0.05) . The level of body weight , renal weight/body weight , SOD and value of ISI in the renal of D and N group were much lower than C group (P<0.05) , however, the markers of N group were higher than D group apparently (P<0.05) .Conclusions: The activities of oxidative stress were enhanced and function of pancreatic islets degenerated apparently in diabetes condition . With the treatment of probucol in early stage , the level of MDA and SOD decreased significantly , meanwhile partially reduced the FPG and restored the pancreatic islets function acting through as an antioxidant . This suggests that oxidative stress may play an important role in the mechanism of type 2diabetes mellitus and diabetic rephropathy.