Expression And Significance Of TGF-β₁,TNF-α In Liver Damage Of Rats Infected With Pagumogonimus Skrjabini

【Objective】To observe the Rats liver pathology characteristic of Pagumogonimus skrjabini disease in hepatic fibrosis and observe the relation of hepatic fibrosis and the expresstion of TGF-β1,TNF-α,In order to reflect the effect of this two factors in the hepatic fibrosis of Pagumogonimus skrjabini disease.To provide the rationale for clinical diagnosis,prophylaxis and treatment.[Methods] Forty two SD rats were divided randomly into two groups:normal animal control group, Infected group. There were 6 rats in the control group,36 rats in the infection group. Raise in the same condition. The animal model were set up by the way of peritoneal injection of Pagumogonimus skrjabini metacercaria to infect rats. Infection group were divided into 6 groups according to infection time:A group (6 rats,infection for 1 week), B group (6 rats,infection for 2 weeks), C group (6 rats,infection for 4 weeks), D group (6 rats,infection for 8 weeks), E group (6 rats, infection for 12 weeks), F group (6 rats,infection for 16 weeks).All rats were killed in corresponding time points after being infected.Before kill the rats,use etherization them and use syringe to draw the blood from rat'jugular and then transfer to 2ml EP.Take. Livers to paraffin section then HE dyeing and VG dyeing,then observe the degree of hepatic fibrosis under the light microscope.Use hydroextractor to ctrifugate the blood, rotation speed is 2000/min,the time is 15 min.Take the serum and use ELISA to detece TGF-β1 and TNF-α. 【Results】The result of HE and VG dyeing:In control group,the results showed liver cells were arranged regularly and the structure of hepatic lobules were integrated in liver tissue. Only there were trifle fibres in the vessel wall,no fiber connective tissue hyperplasia and inflammatory cell infiltration around the portal area.The liver athological change of infected groung show that there were hepatocellular edema in the integrated of the structure of hepatic lobules in A group. in B group, there were extensive fatty degeneration and a spot of balloon-like degeneration of liver cells in the structure of hepatic lobules. there were a large number of inflammatory cells infiltration and a small number of pumctiform necrosis in the central venous and protal area,no fiber fibrous connective tissue hyperplasia by VG staining.in C group,there were more significant balloon-like degeneration,necrosis and inflammatory cell infiltration in the integrated structure of hepatic lobules than B group,and appeared patchy necrosis, a litter of fiber fibrous connective tissue hyperplasia by VG staining.In D group,the obvious chang of pathology was acute purulent inflammation in liver.A mall obscess was formed in the liver tissue. In E group,the flaky fibrous tissue hyperplasia,the some blood essels and small bile duct hyperplasia appear in liver tissue, the structure of some hepatic lobules were damaged,reconstructed, fiber hyperplasia in part of doortude area.hyperplasia fiber were formed cord. To stretch into hepatic lobules. there were minority lymphocytes,neutrophil infiltration in hyperplasia fiber. In F group,a large of liver cells appeared necrosis, hyperplasia fiber were formed cord,the structure of hepatic lobules a were disorder, then appeared further bridging necrosis.The change of TGF-β1is obvious after been infected 1 week in experimental group,after 2 weeks TGF-β1 raise up,after 8 weeks the level is raise up obvious,when it last 16 weeks,it gose to the top.on the same time the level of hepatic fibrosis is also become more and more graveness, TNF-αis growing higher and higher after been infected 1 week,2 weeks and 4 weeks,but the amplification is not so much,after 8 weeks,12 weeks and 16 weeks,the amplification is big and when it gone to 16 weeks,it reached the top point.the expresstion of TNF-αis also become higer and higer. 【Conclusion】To combine the lives pathology change,time and the level of expresstion of TGF-β1 and TNF-αto get the result like that:the liver damagement after been infected Pagumogonimus skrjabini and the level of hepatic fibrosis become more and more graveness along with the time growing. TGF-β1 and TNF-αare two important factors in the process of hepatic fibrosis...